Effects of exogenous GM1 and GD1a on S20Y neuroblastoma cells
Effects of exogenous GM1 and GD1a on S20Y neuroblastoma cells
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外源性GM1和GD1a对S20Y神经母细胞瘤细胞的影响
DOI：
10.1002/jnr.490120106
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发表时间：
1984
影响因子：
4.2
通讯作者：
C. Schengrund
中科院分区：
文献类型：
--
作者：
K. Carine;C. Schengrund;C. Schengrund
The effects of exogenous GM1 and GD1a on S20Y murine neuroblastoma cells were assessed by monitoring morphology, tumorigenicity, mitotic index, and plating efficiency. S20Y cells were seeded at a density equivalent to 5 × 104 cells per 35‐mm tissue culture dish; 38–42 hr after seeding (preconfluent stage) the cells were treated for 12 hr with 100 μg of ganglioside per ml of medium in which the serum content was reduced from 10% to 0.5%. Analysis of the cell lipids indicated that added ganglioside became tightly associated with the membrane during the 12‐hr exposure. GM1 treatment resulted in increased projections on the cell surface and fine structures projecting from the cell processes. GD1a treatment resulted in a reduction in the cellular mitotic index. Plating efficiency was reduced by both GM1 and GD1a. Neither ganglioside affected tumorigenicity of the S20Y cells. Twelve hours after removal of the added ganglioside and exposure of the cells to normal medium, the ganglioside composition of the membranes from treated cells approached that of the controls, and the ganglioside‐induced effects had been reversed. These results suggest that addition of specific gangliosides induces different cellular responses and that these changes are dependent upon the continued presence of the ganglioside.
神经节苷脂 GM1 使肿瘤细胞对生长抑制性糖肽敏感。
DOI：
10.1016/0006-291x(82)91542-x
发表时间：
1982
影响因子：
3.1
作者：
Kinders,RJ;Rintoul,DA;Johnson,TC
通讯作者：
Johnson,TC
神经节苷脂作为受体调节剂。
影响因子：
--
作者：
Bremer,EG;Hakomori,S
通讯作者：
Hakomori,S