SEQUENCE-ANALYSIS OF ENTEROCOCCUS-FAECALIS AGGREGATION SUBSTANCE ENCODED BY THE SEX-PHEROMONE PLASMID PAD1
SEQUENCE-ANALYSIS OF ENTEROCOCCUS-FAECALIS AGGREGATION SUBSTANCE ENCODED BY THE SEX-PHEROMONE PLASMID PAD1
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DOI：
10.1111/j.1365-2958.1990.tb00662.x
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发表时间：
1990-06-01
影响因子：
3.6
通讯作者：
WIRTH, R
中科院分区：
文献类型：
--
作者：
GALLI, D;LOTTSPEICH, F;WIRTH, R
粪肠球菌（Enterococcus faecalis）性信息素质粒pAD 1上的聚集物质结构基因的位置是利用从纯化蛋白的N-末端氨基酸序列推导的寡核苷酸确定的。确定了相应区域的核苷酸序列，并可鉴定出两个开放阅读框（ORF）。ORF 1编码一个小的（Mr 13 160）酸性蛋白，功能未知。发现聚集物质基因（命名为asa 1）编码1296个氨基酸的蛋白质（Mr 142248）。该蛋白质具有43个氨基酸的信号肽（所得的成熟聚集物质的Mr为137 - 429），并在其C-末端区域含有富含脯氨酸的序列，先前表征为参与细胞壁缔合，随后是膜锚。膜锚与其他革兰氏阳性菌的膜锚有显著的相似性，但与革兰氏阳性菌的表面蛋白没有相似性，尤其是pAD 1特异性聚集物质在DNA或蛋白质水平上均未检测到重复。该蛋白质含有Arg-Gly-Asp-Ser和Arg-Gly-Asp-Val（各一个）氨基酸基序，据推测，它们在粘附真核细胞中起着至关重要的作用。
The location of the structural gene for aggregation substance on the sex pheromone plasmid pAD1 of Enterococcus faecalis was determined using an oligonucleotide deduced from the N-terminal amino acid sequence of the purified protein. The nucleotide sequence was determined for the corresponding region and two open reading frames (ORFs) could be identified. ORF1 codes for a small (Mr 13 160) acidic protein of unknown function. The gene for aggregation substance (names asa1) was found to code for a protein of 1296 amino acids (Mr 142248). The protein has a signal peptide of 43 amino acids (the resulting Mr for mature aggregation substance is 137 429) and contains in its C-terminal region a proline-rich sequence, previously characterized as being involved in cell wall association, which is followed by a membrane anchor. The membrane anchor showed significant similarity to that of other Gram-positive organisms, but no other similarities to surface proteins from Gram-positive bacteria were found. In particular, no repeats on the DNA or protein level could be detected for pAD1-specific aggregation substance. The protein contains the amino acid motifs Arg-Gly-Asp-Ser and Arg-Gly-Asp-Val (once each), which, it is proposed, play a crucial role in adherence to eukaryotic cells.