A spatially varying charge model for regulating site-selective protein adsorption and cell behaviors
A spatially varying charge model for regulating site-selective protein adsorption and cell behaviors
复制标题
用于调节位点选择性蛋白质吸附和细胞行为的空间变化电荷模型
DOI：
10.1039/c8bm01158c
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发表时间：
2019
影响因子：
6.6
通讯作者：
Ning Chengyun
中科院分区：
文献类型：
--
作者：
Zhang Kejia;Xing Jun;Chen Junqi;Wang Zhengao;Zhai Jinxia;Yao Tiantian;Tan Guoxin;Qi Suijian;Chen Dafu;Yu Peng;Ning Chengyun
进入体内的植入材料首先与体液中的蛋白质相互作用，然后细胞通过这层吸附的蛋白质感知并响应外来植入物。因此，蛋白质吸附在植入物表面上的空间特异性调节对于介导随后的细胞行为至关重要。与传统生物材料的表面调制策略不同，在本研究中，设计了具有非均匀表面电荷空间分布的材料，以实现位点选择性蛋白质吸附，并通过电荷调节进一步影响细胞行为。通过聚焦激光束诱导相变产生具有不同压电水平的空间变化的微畴。此外，极化后，具有不同水平的压电性的区域的表面电荷密度显示出显着差异。扫描开尔文探针力显微镜（SKPM）的结果表明，在激光照射和极化后，材料表面电荷呈现出不均匀的空间分布。通过蛋白质吸附实验证明了位点特异性电荷介导的选择性蛋白质吸附。细胞行为分析表明，电荷密度的增加有利于促进细胞粘附和丝状伪足的形成，而电荷空间分布的不均匀性则促进了细胞的定向排列，这两个特征都加速了细胞的迁移。该研究为通过表面电荷空间调控蛋白质吸附进而影响细胞行为提供了一种新的方法。
Implanted materials that enter the body first interact with proteins in body fluids, and cells then perceive and respond to the foreign implant through this layer of adsorbed proteins. Thus, spatially specific regulation of protein adsorption on an implant surface is pivotal for mediating subsequent cellular behaviors. Unlike the surface modulation strategy for traditional biomaterials, in this research, materials with a nonuniform spatial distribution of surface charges were designed to achieve site-selective protein adsorption and further influence cell behavior by charge regulation. Spatially varying microdomains with different levels of piezoelectricity were generated via a focus laser beam-induced phase transition. In addition, after polarization, the zones with different levels of piezoelectricity showed significant differences in surface charge density. The results of scanning Kelvin probe force microscopy (SKPM) showed that the surface charge on the material exhibits a nonuniform spatial distribution after laser irradiation and polarization. Site-specific charge-mediated selective protein adsorption was demonstrated through a protein adsorption experiment. Cell behavior analysis showed that the increase in charge density was conducive to promoting cell adhesion and the formation of filopodia while the nonuniform spatial distribution of charge promoted an oriented arrangement of cells; both features accelerated cell migration. This study provides a new method for spatially regulating protein adsorption through surface charges to further influence cell behaviors.