Analysis of catalytic residues in enzyme active sites
Analysis of catalytic residues in enzyme active sites
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DOI：
10.1016/s0022-2836(02)01036-7
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发表时间：
2002-11-15
影响因子：
5.6
通讯作者：
Thornton, JM
中科院分区：
文献类型：
--
作者：
Bartlett, GJ;Porter, CT;Thornton, JM
我们提出了一个分析的残基直接参与催化在178个酶的活性位点。推导出定义催化残留物的具体标准，并用于创建催化残留物数据集，然后根据包括二级结构、溶剂可及性、灵活性、保守性、四级结构和功能在内的性质对其进行分析。结果表明，一小组的氨基酸残基在催化中的优势，并给出了一个一般的活性位点环境的图片。希望这些信息将提供一个更好的理解参与催化的分子机制和预测未知功能的酶的催化残基的启发式基础。(C)2002爱思唯尔科技有限公司版权所有。
We present an analysis of the residues directly involved in catalysis in 178 enzyme active sites. Specific criteria were derived to define a catalytic residue, and used to create a catalytic residue dataset, which was then analysed in terms of properties including secondary structure, solvent accessibility, flexibility, conservation, quaternary structure and function. The results indicate the dominance of a small set of amino acid residues in catalysis and give a picture of a general active site environment. It is hoped that this information will provide a better understanding of the molecular mechanisms involved in catalysis and a heuristic basis for predicting catalytic residues in enzymes of unknown function. (C) 2002 Elsevier Science Ltd. All rights reserved.