Detection of the reelin breakpoint in reeler mice
Detection of the reelin breakpoint in reeler mice
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DOI：
10.1016/0169-328x(96)00046-0
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发表时间：
1996-07-01
期刊：
影响因子：
--
通讯作者：
Curran, T
中科院分区：
文献类型：
--
作者：
DArcangelo, G;Miao, GG;Curran, T
通过部分缺失破坏reelin基因导致称为reeler的神经学表型。在这里，我们报告了从杰克逊reeler株（rl）的reelin断裂点区域的克隆和测序。基于此序列，我们开发了一种聚合酶链反应筛选，允许在表型出现之前识别突变小鼠。该测定还允许区分杂合小鼠和野生型小鼠。这些研究结果提供了一个战略的reeler突变的早期解剖和生理后果的表征。
Disruption of the reelin gene by partial deletion causes the neurological phenotype known as reeler. Here we report the cloning and sequencing of the reelin breakpoint region from the Jackson reeler strain (rl). Based on this sequence, we developed a polymerase chain reaction screen that allows the identification of mutant mice prior to the appearance of the phenotype. The assay also permits discrimination of heterozygous from wild-type mice. These findings provide a strategy for the characterization of the early anatomical and physiological consequences of the reeler mutation.