Ga1α(1,3) Ga1 epitope in porcine small intestinal submucosa
Ga1α(1,3) Ga1 epitope in porcine small intestinal submucosa
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DOI：
10.1089/10763270050044416
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发表时间：
2000-06-01
影响因子：
--
通讯作者：
Badylak, SF
中科院分区：
文献类型：
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作者：
McPherson, TB;Liang, H;Badylak, SF
小肠黏膜下层(SIS)是一种来源于猪空肠的天然无细胞生物材料，作为异种移植材料可促进结构性组织重塑。半乳糖基-α(1，3)半乳糖(Gal)是灵长类动物异种猪全器官移植超急性排斥反应的细胞相关表位。因为SIS是从猪组织中提取的，所以它可能含有Gal表位。这项研究的目的是确定SIS中是否存在Gal，如果存在，则确定暴露于猪源SIS后，人血清补体是否可以在体外被激活。用免疫组织化学方法和凝集素-过氧化物酶染色检测SIS中Gal的表达。SIS与含有自然产生的Gal抗体的人血清呈强阳性反应，其次是抗免疫球蛋白G(Ig G)或抗Ig M过氧化物酶结合物。用Gal表位特异的凝集素I-B-4阻断，降低了染色强度。将SIS暴露于α-半乳糖苷酶可使染色减少到微不足道的程度。Gal表位跨壁分布在整个SIS材料中。对与SIS结合的免疫球蛋白进行亚型分析表明，Ig G(2)是人血浆中与SIS结合的主要免疫球蛋白。用C3a放射免疫法测定，SIS在体外不能激活补体。
Small intestinal submucosa (SIS) is a naturally occurring, acellular biomaterial derived from porcine jejunum, which promotes constructive tissue remodeling when applied as a xenogeneic graft material. Galactosyl-alpha(1,3)galactose (Gal) is a cell-associated epitope responsible for hyperacute rejection of porcine whole-organ xenografts in primates. Because SIS is harvested from porcine tissue, it may contain the Gal epitope. The goals of this study were to determine if Gal is present in SIS and, if it is present, to determine if human serum complement can be activated in vitro following exposure to porcine-derived SIS. SIS was probed for Gal by immunohistochemical methods and by lectin-peroxidase staining. SIS stained strongly positive with human serum, which contains naturally occurring antibodies to Gal, followed by anti-immunoglobulin G (IgG) or anti-IgM peroxidase conjugate. Blocking with the lectin I-B-4, which is specific for the Gal epitope, decreased the intensity of staining. Exposure of SIS to alpha-galactosidase reduced staining to negligible amounts. The Gal epitope is distributed transmurally throughout the SIS material. Subtyping of the immunoglobulins that bind to SIS showed that IgG(2) is the major immunoglobulin of human plasma that binds to SIS. SIS did not activate complement in vitro as measured by radioimmunoassay for C3a.