THE ADSORPTION OF DIVALENT-CATIONS TO PHOSPHATIDYLGLYCEROL BILAYER-MEMBRANES
THE ADSORPTION OF DIVALENT-CATIONS TO PHOSPHATIDYLGLYCEROL BILAYER-MEMBRANES
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DOI：
10.1016/0005-2736(81)90199-1
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
MCLAUGHLIN, S
中科院分区：
文献类型：
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作者：
LAU, A;MCLAUGHLIN, A;MCLAUGHLIN, S
结合31 P-NMR和电泳迁移率测量的斯特恩方程来描述二价阳离子的磷脂酰甘油膜的吸附的能力进行了测试。在0.1 M NaCl中，31 P-NMR和zeta电位数据均由Stern方程很好地描述。~（31）P-NMR和~（13）C-NMR结果表明，钴只与磷脂酰甘油分子的磷酸基团形成内球络合物，而大部分吸附的钴离子形成外球络合物。有证据表明，碱土金属阳离子也结合磷脂主要是通过形成外层复合物。用几种不同的二价阳离子进行电泳迁移率测量。在所有情况下，在0.1 M NaCl的zeta电位很好地描述了斯特恩方程。磷脂酰甘油复合物的固有1：1缔合常数（M-1）依次降低：Mn 2+，11.5; Ca 2+，8.5; Ni 2+，7.5; Co 2+，6.5; Mg 2+，6.0; Ba 2+，5.5和Sr 2+，5.0。
The ability of the Stern equation to describe the adsorption of divalent cations to phosphatidylglycerol membranes was tested by combining 31P-NMR and electrophoretic mobility measurements. In 0.1 M NaCl both the 31P-NMR and the zeta potential data are well described by the Stern equation. 31P-NMR and 13C-NMR results indicate that cobalt forms inner-sphere complexes only with the phosphate group of phosphatidylglycerol molecules and that a substantial fraction of the adsorbed Co ions form outer-sphere complexes. Evidence is presented that suggests the alkaline earth cations also bind to phospholipids mainly by forming outer sphere complexes. Electrophoretic mobility measurements were performed with several different divalent cations. In all cases the zeta potentials in 0.1 M NaCl were well described by the Stern equation. The intrinsic 1:1 association constants (M-1) for the phosphatidylglycerol complexes decreased in the sequence: Mn2+, 11.5; Ca2+, 8.5; Ni2+, 7.5; Co2+, 6.5; Mg2+, 6.0; Ba2+, 5.5 and Sr2+, 5.0.