The effects of suspending medium viscosity on erythrocyte deformation and haemolysis in vitro.
The effects of suspending medium viscosity on erythrocyte deformation and haemolysis in vitro.
复制标题
悬浮介质粘度对体外红细胞变形和溶血的影响。
DOI：
10.1016/0005-2736(79)90215-3
复制
发表时间：
1979
期刊：
影响因子：
--
通讯作者：
A. R. Williams
中科院分区：
文献类型：
--
作者：
D. Morris;A. R. Williams
新鲜的成人红细胞悬浮在含有不同浓度右旋糖酐T.500的等渗pH调节溶液中。细胞在费兰蒂雪莉锥和平板粘度计中受到均匀的水动剪切应力。在任何给定的暴露参数组合下产生的裂解量受悬浮介质粘度的显著影响。当细胞经历剪切时获得的光学衍射图表明，细胞变形也是粘度的函数。因此，受压细胞的扭曲形状在溶血过程中起着至关重要的作用。
Fresh adult human erythrocytes were suspended in isotonic pH adjusted solutions containing various concentrations of Dextran T.500. The cells were subjected to uniform hydrodynamic shear stress in a Ferranti Shirley Cone and Plate Viscosimeter. The amount of lysis incurred at any given combination of explosure parameters was markedly affected by the viscosity of the suspending medium. Optical diffraction patterns obtained whilst the cells were undergoing shear demonstrated that cellular deformation was also a function of viscosity. Consequently, the distorted shape of the stressed cell must play a crucial role in the haemolytic process.