Site-Selective Nucleation and Size Control of Gold Nanoparticle Photothermal Antennae on the Pore Structures of a Virus
Site-Selective Nucleation and Size Control of Gold Nanoparticle Photothermal Antennae on the Pore Structures of a Virus
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DOI：
10.1021/jacs.8b10446
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发表时间：
2018-12-12
影响因子：
15
通讯作者：
Gassensmith, Jeremiah J.
中科院分区：
文献类型：
--
作者：
Benjamin, Candace E.;Chen, Zhuo;Gassensmith, Jeremiah J.
在这篇文章中，我们表明噬菌体Q β的表面配备了天然配体，用于合成小金纳米颗粒（AuNPs）。通过利用蛋白质二级结构中的二硫化物和衣壳四级结构形成的几何形状，我们发现我们可以在病毒样颗粒的表面上产生类似于6 nm AuNP的规则排列的图案。实验和计算分析提供了深入了解这种复合材料的形成和稳定性。我们进一步表明，包埋的遗传物质可以容纳500个抗癌药物阿霉素分子，而不会泄漏，也不会干扰金纳米颗粒的合成。衣壳上的纳米颗粒的这种直接成核允许在纳秒激光照射时光热能量的异常传导。作为原理的证明，我们证明了这种能量能够在不加热本体溶液的情况下从衣壳中快速释放药物，从而在体外实现高度靶向的细胞杀伤。
In this Article, we show that the surface of the bacteriophage Q beta is equipped with natural ligands for the synthesis of small gold nanoparticles (AuNPs). By exploiting disulfides in the protein secondary structure and the geometry formed from the capsid quaternary structure, we find that we can produce regularly arrayed patterns of similar to 6 nm AuNPs across the surface of the virus-like particle. Experimental and computational analyses provide insight into the formation and stability of this composite. We further show that the entrapped genetic material can hold upward of 500 molecules of the anticancer drug Doxorubicin without leaking and without interfering with the synthesis of the AuNPs. This direct nucleation of nanoparticles on the capsid allows for exceptional conduction of photothermal energy upon nanosecond laser irradiation. As a proof of principle, we demonstrate that this energy is capable of rapidly releasing the drug from the capsid without heating the bulk solution, allowing for highly targeted cell killing in vitro.