GENOMIC TERMINI OF EQUINE HERPESVIRUS-1
GENOMIC TERMINI OF EQUINE HERPESVIRUS-1
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DOI：
10.1128/jvi.64.2.873-880.1990
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发表时间：
1990-02-01
影响因子：
5.4
通讯作者：
HAMMERSCHMIDT, W
中科院分区：
文献类型：
--
作者：
CHOWDHURY, SI;BUHK, HJ;HAMMERSCHMIDT, W
马疱疹病毒1型（EHV-1）感染细胞后，线性双链DNA基因组的末端融合形成环状。为了研究这种复制型DNA的产生和切割机制，已经通过限制性作图、印迹杂交、克隆和测序分析了基因组末端、来自复制型分子的末端融合以及短基因组片段和长基因组片段之间的连接。数据表明，基因组末端不是冗余的，并且基因组末端通过独特长片段（UL）和末端重复序列（TR）末端的3“单碱基延伸融合在复制中间体中。与EHV-1末端相邻的是AT和γ。在不同疱疹病毒中高度保守的序列元件。我们认为这两个序列元件对EHV-1复制型的裂解都很重要。
After cell infection with the equine herpesvirus 1 (EHV-1), the termini of the linear double-stranded DNA genome fuse to form circular forms. To investigate the mechanisms in the generation and cleavage of such replicative-from DNAs, the genomic termini, the fusion of termini from replicative-form molecules, and the junction between the short and long genome segments have been analyzed by restriction mapping, blot hybridizations, cloning, and sequencing. The data suggest that the genome ends are not redundant and that the genomic termini are fused in replicative intermediates via 3'' single-base extensions at the termini of the unique long segment (UL) and terminal repeat (TR). Adjacent to the EHV-1 termini are AT and .gamma. sequence elements highly conserved among different herpesviruses. We propose that both of these sequence elements are important for the cleavage of EHV-1 replicative forms.