Gamma interferon induces monocytoid differentiation in the HL-60 cell line.
Gamma interferon induces monocytoid differentiation in the HL-60 cell line.
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γ 干扰素诱导 HL-60 细胞系中的单核细胞分化。
DOI：
10.1172/jci111292
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Fanger,MW
中科院分区：
文献类型：
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作者：
Ball,ED;Guyre,PM;Shen,L;Glynn,JM;Maliszewski,CR;Baker,PE;Fanger,MW
我们研究了纯化的重组DNA来源的干扰素(干扰素)诱导HL-60早幼粒白血病细胞系表型变化的能力。监测单抗检测到的细胞表面标志物的变化以及形态、组织化学和功能的变化。我们发现，γ-干扰素诱导单核细胞和粒细胞(AML-2-23、63D3和61D3)特有的抗原表达，以及符合单核细胞分化的形态变化，而不是α-或β-干扰素。这些包括α-乙酸萘酯酶的诱导，细胞大小的增加，以及亲天青颗粒的减少。γ-干扰素对抗原表达的影响具有剂量依赖性和时程效应，提示γ-干扰素可诱导从头合成蛋白质。用抗γ-干扰素的单抗阻断γ-干扰素的活性和混合淋巴细胞培养上清的活性。γ-干扰素可显著增强HL-60细胞介导抗体依赖性细胞毒作用的能力。这些发现表明，γ-干扰素在造血调节中起作用。
We investigated the ability of purified, recombinant DNA-derived interferons (IFN) to induce phenotypic changes in cells of the HL-60 promyelocytic leukemia cell line. Changes in cell surface markers detected by monoclonal antibodies as well as morphologic, histochemical, and functional changes were monitored. We found that gamma-IFN, but not alpha- or beta-IFN, induced the expression of antigens characteristic of monocytes and granulocytes (AML-2-23, 63D3, and 61D3), as well as changes in morphology consistent with monocytoid differentiation. These included induction of alpha-naphthyl acetate esterase, increased cell size, and a decrease in azurophilic granules. The gamma-IFN dose dependency and time course of the effect on antigen expression suggest that de novo protein synthesis was induced by gamma-IFN. The activity of gamma-IFN and of mixed-lymphocyte culture supernatant was blocked by a monoclonal antibody to gamma-IFN. Significant augmentation in the ability of the HL-60 cells to mediate antibody-dependent cellular cytotoxicity was induced by gamma-IFN. These findings suggest that gamma-IFN plays a role in the regulation of hematopoiesis.