Creatine and tempol attenuate noise-induced hearing loss
Creatine and tempol attenuate noise-induced hearing loss
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DOI：
10.1016/j.brainres.2007.02.021
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发表时间：
2007-05-07
期刊：
影响因子：
2.9
通讯作者：
Miller, Josef M.
中科院分区：
文献类型：
--
作者：
Minami, Shujiro B.;Yamashita, Daisuke;Miller, Josef M.
为了确定自由基形成和潜在能量消耗在噪声性听力损失（NIHL）中的作用，我们测量了tempol（自由基清除剂）和肌酸（增强细胞能量储存）单独和联合使用对NIHL的减弱效果。豚鼠被分成四个治疗组:控制,3%的肌酸饮食(噪声暴露前2周),tempol(3毫米饮用水接触前2周),肌酸+ tempol和暴露在120分贝噪音一个八度带集中在4 kHz 5 h。noise-only对照组显示频率相关听阈变化(以听觉脑干反应来衡量,ABR)高达73分贝(16千赫)1天,和50 dB在10天(8 kHz)。在第1天和第10天，C维丁处理的受试者ABR阈值变化明显较小。Tempol在第10天显著减少ABR阈值变化，但在第1天没有。联合治疗后ABR变化与肌酸组相似。第10天，肌酸和天酚单独或联合使用均能减轻毛细胞损失。我们的研究结果表明，维持ATP水平对减轻暂时性和永久性NIHL都很重要，而清除自由基则提供永久性NIHL的保护。(c) 2007 Elsevier B.V.版权所有
To define the role of free radical formation and potential energy depletion in noise induced hearing loss (NIHL), we measured the effectiveness of tempol (free radical scavenger) and creatine (enhances cellular energy storage) alone and in combination to attenuate NIHL. Guinea pigs were divided into four treatment groups: controls, 3% creatine diet (2 weeks prior to noise exposure), tempol (3 mM in drinking water 2 weeks prior to exposure), and creatine plus tempol and exposed to 120 dB SPL one-octave band noise centered at 4 kHz for 5 h. The noise-only control group showed frequency-dependent auditory threshold shifts (measured by auditory brainstem response, ABR) of up to 73 dB (16 kHz) on day 1, and up to 50 dB (8 kHz) on day 10. C reatine-treated subjects had significantly smaller ABR threshold shifts on day 1 and on day 10. Tempol alone significantly reduced ABR threshold shifts on day 10 but not on day 1. ABR shifts after combination treatment were similar to those in the creatine group. Hair cell loss on day 10 was equally attenuated by creatine and tempol alone or in combination. our results indicate that the maintenance of ATP levels is important in attenuating both temporary and permanent NIHL, while the scavenging of free radicals provides protection from permanent NIHL. (c) 2007 Elsevier B.V. All rights reserved.