AUTO-REGULATION OF FOS - THE DYAD SYMMETRY ELEMENT AS THE MAJOR TARGET OF REPRESSION
AUTO-REGULATION OF FOS - THE DYAD SYMMETRY ELEMENT AS THE MAJOR TARGET OF REPRESSION
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DOI：
10.1002/j.1460-2075.1989.tb08394.x
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发表时间：
1989-09-01
期刊：
影响因子：
11.4
通讯作者：
HERRLICH, P
中科院分区：
文献类型：
--
作者：
KONIG, H;PONTA, H;HERRLICH, P
Fos和Jun协同抑制FOS启动子。从FOS启动子上移除所有假定的Fos/Jun结合位点既不能消除Fos/Jun在NIH3T3细胞瞬时共转染实验中的抑制作用，也不能关闭血清诱导稳定表达的NIH3T3细胞中Fos表达的动力学。二分体对称元件(DSE)足以使启动子受到这种类型的抑制。然而，其中一个假定的Fos/Jun结合位点(-292到-299，因此紧挨着DSE)决定了非常低的基础表达水平。
Fos and Jun co‐operatively repress the fos promoter. Removal of all putative Fos/Jun binding sites from the fos promoter neither obliterates the repression by Fos/Jun in transient cotransfection experiments in NIH3T3 cells nor the turn‐off kinetics of serum‐induced fos expression in stably transfected NIH3T3 cells. The dyad symmetry element (DSE) suffices to subject a promoter to this type of repression. However, one of the putative Fos/Jun binding sites (‐292 to ‐299 and thus located immediately adjacent to the DSE), determines the very low level of basal expression.