Optogenetic analysis of synaptic function
Optogenetic analysis of synaptic function
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DOI：
10.1038/nmeth.1252
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发表时间：
2008-10-01
期刊：
影响因子：
48
通讯作者：
Gottschalk, Alexander
中科院分区：
文献类型：
--
作者：
Liewald, Jana F.;Brauner, Martin;Gottschalk, Alexander
我们介绍了神经传递的光遗传学研究（OptIoN），用于通过行为和电生理分析对突触功能进行时间分辨和定量评估。我们使用莱茵衣藻视紫红质-2的靶向表达，在秀丽隐杆线虫神经肌肉接头处光触发乙酰胆碱或γ-氨基丁酸的释放。在完整的视紫红质-2转基因蠕虫，光刺激立即诱导身体伸长（γ-氨基丁酸）或收缩（乙酰胆碱），我们分析了急性，或在持续激活自动图像分析，以评估突触的功效。在解剖的蠕虫中，光刺激诱发了神经递质特异性突触后电流，这种电流可以以不同的频率重复触发。光诱发行为和突触后电流显著增加（P
We introduce optogenetic investigation of neurotransmission (OptIoN) for time-resolved and quantitative assessment of synaptic function via behavioral and electrophysiological analyses. We photo-triggered release of acetylcholine or gamma-aminobutyric acid at Caenorhabditis elegans neuromuscular junctions using targeted expression of Chlamydomonas reinhardtii Channelrhodopsin-2. In intact Channelrhodopsin-2 transgenic worms, photostimulation instantly induced body elongation (for gamma-aminobutyric acid) or contraction ( for acetylcholine), which we analyzed acutely, or during sustained activation with automated image analysis, to assess synaptic efficacy. In dissected worms, photostimulation evoked neurotransmitter-specific postsynaptic currents that could be triggered repeatedly and at various frequencies. Light-evoked behaviors and postsynaptic currents were significantly (P