Characterization and Genome Analysis of a Zearalenone-Degrading Bacillus velezensis Strain ANSB01E
Characterization and Genome Analysis of a Zearalenone-Degrading Bacillus velezensis Strain ANSB01E
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玉米赤霉烯酮降解芽孢杆菌菌株 ANSB01E 的表征和基因组分析
DOI：
10.1007/s00284-019-01811-8
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发表时间：
2020-02-01
影响因子：
2.6
通讯作者：
Zhao, Lihong
中科院分区：
文献类型：
--
作者：
Guo, Yongpeng;Zhou, Jianchuan;Zhao, Lihong
玉米赤霉烯酮是一种主要由镰刀菌属真菌产生的非甾体类雌激素类真菌毒素，可引起动物和人类的生殖障碍和高雌激素综合征。从鸡盲肠内容物中分离到一株能有效降解玉米赤霉烯酮的细菌Bacillus velezensis ANSB 01 E。此外，Velezensis ANSB 01 E对包括大肠杆菌、金黄色葡萄球菌和沙门氏菌在内的动物病原菌具有良好的抗菌活性。基于基因组的分析揭示了在Velezensis ANSB 01 E中存在编码过氧化物氧还蛋白和α/β水解酶的基因，其可能参与玉米赤霉烯酮的降解。基因组的研究为深入了解玉米赤霉烯酮的降解机理提供了新的思路，并为进一步研究其在食品和饲料工业中的应用提供了新的思路。
Zearalenone, a nonsteroidal estrogenic mycotoxin mainly produced by Fusarium species, causes reproductive disorders and hyperestrogenic syndromes in animals and humans. The bacterial strain Bacillus velezensis ANSB01E, isolated from chicken cecal content, was capable of effectively degrading zearalenone in both liquid medium and mouldy corn. Moreover, Bacillus velezensis ANSB01E exhibited good antimicrobial activities against animal pathogenic bacteria, including Escherichia coli, Staphylococcus aureus, and Salmonella spp. Genome-based analysis revealed the presence of genes coding peroxiredoxin and alpha/beta hydrolase in Bacillus velezensis ANSB01E, which may be involved in zearalenone degradation. The study on the genome provides insights into the zearalenone degradation mechanisms and advances the potential application of Bacillus velezensis ANSB01E in food and feed industry.