Silk fibroin as a water-soluble bio-resist and its thermal properties
Silk fibroin as a water-soluble bio-resist and its thermal properties
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DOI：
10.1039/c5ra20201a
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发表时间：
2016-01-01
期刊：
影响因子：
3.9
通讯作者：
Juodkazis, Saulius
中科院分区：
文献类型：
--
作者：
Morikawa, Junko;Ryu, Meguya;Juodkazis, Saulius
用直接接触法测定了丝素蛋白膜的热扩散系数α =（1.6 +/-0.24）× 10（-7）m（2）s（-1）。在结晶相中，它被显示为降低到接近1 × 10（-7）m（2）s（-1），与β-折叠组装体的多域组成一致。通过浸渍到酒精中制成具有β片层的结晶丝，并将其用作正电子束光刻（EBL）抗蚀剂。通过1619 cm（-1）酰胺-I C=O光谱特征和3290 cm（-1）酰胺-A N-H伸缩带的直接IR成像显示，电子束负责解链β-片层，其随后导致暴露区域返回到水溶性状态。这使得有可能开发一种水基生物相容性丝抗蚀剂，并将其用于光刻应用。蛋白质结晶的一般原理，可追溯到丝的IR酰胺谱带的光谱变化，可用作创建新的蛋白质EBL抗蚀剂的指导，并量化溶解度所需的电子剂量。丝的泡沫形成和激光处理可以为表面功能化和三维生物支架的制备提供新的途径。
Thermal diffusivity of silk fibroin films, alpha = (1.6 +/- 0.24) x 10(-7) m(2) s(-1), was measured by a direct contact method. It was shown to be reduced down to similar to 1 x 10(-7) m(2) s(-1) in the crystallized phase, consistent with the multi-domain composition of beta-sheet assemblies. Crystalline silk with beta-sheets was made by dipping into alcohol and was used as a positive electron beam lithography (EBL) resist. It is shown by direct IR imaging of the 1619 cm(-1) amide-I C=O spectral signature and 3290 cm(-1) amide-A N-H stretching band that an e-beam is responsible for unzipping beta-sheets, which subsequently results in exposed areas returning to a water soluble state. This makes it possible to develop a water-based biocompatible silk resist and use it in lithography applications. The general principles of protein crystallization, traceable to spectral changes in IR amide bands of silk, can be used as a guide for the creation of new protein EBL resists and to quantify the electron dose required for solubility. Foam formation and laser treatments of silk can provide new approaches in surface functionalization and fabrication of 3D bio-scaffolds.