Enhancement of equine infectious anemia virus virulence by identification and removal of suboptimal nucleotides
Enhancement of equine infectious anemia virus virulence by identification and removal of suboptimal nucleotides
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DOI：
10.1016/s0042-6822(03)00351-9
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发表时间：
2003-09-01
期刊：
影响因子：
3.7
通讯作者：
Issel, CJ
中科院分区：
文献类型：
--
作者：
Cook, RF;Cook, SJ;Issel, CJ
马传染性贫血病毒（EIAV）的致病性变异株（EIAV（PV））的3 '端序列被置换后，其致病性被恢复。然而，发现嵌合克隆（EIAV（UK））的马/矮种马的发病率显著低于（P = 0.016）EIAV（PV）。这归因于前病毒基因组的3 '而不是5'区域，其中EIAV（UK）与共有EIAV（PV）序列的不同之处在于在3 'LTR和精氨酸（R，03）而不是Rev第二个外显子第103位的色氨酸（W-103）。在EIAV（英国）受体中，重复被迅速消除，R-103被W-取代103在病毒人群中。此外，从EIAV（UK）（EIAV（UK3））中去除3 '变体序列导致马中与EIAV（PV）相当（P = 0.013）的疾病潜力。68-bp重复和/或R-103可能限制急性感染期间病毒RNA积累的峰值。(C)2003 Elsevier Science（美国）。All rights reserved.
Pathogenicity was reportedly restored to an a virulent molecular clone of equine infectious anemia virus (EIAV) by substitution of 3' sequences from the pathogenic variant strain (EIAV(PV)). However, the incidence of disease in horses/ponies was found to be significantly lower (P = 0.016) with the chimeric clone (EIAV(UK)) than with EIAV(PV). This was attributable to 3' rather than 5' regions of the proviral genome, where EIAV(UK) differs from the consensus EIAV(PV) sequence by having a 68-bp duplication in the 3' LTR and arginine (R, 03) rather than tryptophan (W-103) at position 103 in the second exon of rev. In EIAV(UK) recipients the duplication was rapidly eliminated and R-103 replaced by W-103 in the viral population. Furthermore, removal of the 3' variant sequences from EIAV(UK) (EIAV(UK3)) resulted in an equivalent (P = 0.013) disease potential in Equus caballus to EIAV(PV). The 68-bp duplication and/or R-103 may limit peak viral RNA accumulation during acute infection. (C) 2003 Elsevier Science (USA). All rights reserved.