Assessing oxidative stress induction ability and oxidative potential of PM2.5 in cities in eastern and western Japan
Assessing oxidative stress induction ability and oxidative potential of PM2.5 in cities in eastern and western Japan
复制标题
评估日本东部和西部城市PM2.5的氧化应激诱导能力和氧化潜力
DOI：
10.1016/j.chemosphere.2023.138308
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发表时间：
2023
期刊：
影响因子：
8.8
通讯作者：
Kajino Mizuo
中科院分区：
文献类型：
--
作者：
Fujitani Yuji;Furuyama Akiko;Hayashi Masahiko;Hagino Hiroyuki;Kajino Mizuo
氧化应激是与暴露于PM2.5有关的呼吸系统疾病的重要原因。因此，评估PM2.5氧化潜力(OP)的非细胞方法已被广泛评估，用作生物体内氧化应激的指标。然而，基于OP的评估只反映了颗粒的物理化学性质，而没有考虑颗粒与细胞之间的相互作用。因此，为了确定OP在不同PM2.5情景下的效力，我们使用基于细胞的方法--血红素加氧酶-1(HO-1)--进行了氧化应激诱导能力(OSIA)的评估，并将结果与使用脱细胞方法--二硫苏糖醇-1法的OP测量结果进行了比较。在这些检测中，在日本的两个城市收集了PM2.5过滤器样本。为了定量确定PM2.5中金属量和有机气溶胶(OA)亚型对OSIA和OP的相对贡献，还进行了在线测量和离线化学分析。研究结果显示，对于水提取的样品，OSIA与OP之间存在正相关关系，证实OP通常很适合用作OSIA的指标。然而，对于水溶(WS)-铅含量较高的样品，两种分析方法之间的对应程度不同，其OSIA高于其他样品的OP预期。试剂-溶液实验结果表明，在15分钟的反应中，WS-Pb诱导了OSIA，而不是OP，这表明两种检测方法在样品之间存在不一致的关系。多元线性回归分析和试剂-溶液实验表明，WS、过渡金属和生物质燃烧OA分别占水提取PM2.5样品总OSIA或总OP的30%~40%和50%。这是第一次评估HO-1试验评估的细胞氧化应激与不同的骨性关节炎亚型之间的联系。
Oxidative stress is an important cause of respiratory diseases associated with exposure to PM2.5. Accordingly, acellular methods for assessing the oxidative potential (OP) of PM2.5have been evaluated extensively for use as indicators of oxidative stress in living organisms. However, OP-based assessments only reflect the physicochemical properties of particles and do not consider particle-cell interactions. Therefore, to determine the potency of OP under various PM2.5scenarios, oxidative stress induction ability (OSIA) assessments were performed using a cell-based method, the heme oxygenase-1 (HO-1) assay, and the findings were compared with OP measurements obtained using an acellular method, the dithiothreitol assay. For these assays, PM2.5filter samples were collected in two cities in Japan. To quantitatively determine the relative contribution of the quantity of metals and subtypes of organic aerosols (OA) in PM2.5to the OSIA and the OP, online measurements and offline chemical analysis were also performed. The findings showed a positive relationship between the OSIA and OP for water-extracted samples, confirming that the OP is generally well suited for use as an indicator of the OSIA. However, the correspondence between the two assays differed for samples with a high water-soluble (WS)–Pb content, which had a higher OSIA than would be expected from the OP of other samples. The results of reagent-solution experiments showed that the WS-Pb induced the OSIA, but not the OP, in 15-min reactions, suggesting a reason for the inconsistent relationship between the two assays across samples. Multiple linear regression analyses and reagent-solution experiments showed that WS transition metals and biomass burning OA accounted for approximately 30–40% and 50% of the total OSIA or the total OP of water-extracted PM2.5samples, respectively. This is the first study to evaluate the association between cellular oxidative stress assessed by the HO-1 assay and the different subtypes of OA.