The suppression of zfpm-1 accelerates the erythropoietic differentiation of human CD34+ cells.
The suppression of zfpm-1 accelerates the erythropoietic differentiation of human CD34+ cells.
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DOI：
10.1016/j.bbrc.2006.12.155
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发表时间：
2007-02
影响因子：
3.1
通讯作者：
Hee-Young Yang;Suehee Kim;Seok-Ho Kim;Dong-Jun Kim;Sun-Uk Kim;Dae-Yeul Yu;Y. Yeom;Dong-Seok Lee;Yong-jun Kim;Byung-Ju Park;Tae-Hoon Lee
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文献类型：
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作者：
Hee-Young Yang;Suehee Kim;Seok-Ho Kim;Dong-Jun Kim;Sun-Uk Kim;Dae-Yeul Yu;Y. Yeom;Dong-Seok Lee;Yong-jun Kim;Byung-Ju Park;Tae-Hoon Lee
红细胞生成是从造血干细胞分化为成熟红细胞的复杂多阶段过程。已有报道几种转录因子在造血干细胞分化中的作用。然而，控制其功能行为的分子基础尚不清楚。在这项研究中，我们的特点Zfpm-1在人造血干细胞的红细胞分化的作用。为了验证Zfpm-1在红细胞分化中的作用，我们利用人脐带血建立了人CD 34+细胞培养体系。在人CD 34+细胞分化为原代细胞过程的第7天，Zfpm-1最初上调，然后在第9天显著下调。Zfpm-1 siRNA转染的HSC比模拟转染的细胞含有多20%的GPA+细胞，并且显示造血转录因子c-myc和c-myb的表达受到抑制，但加塔-1的表达增加。相比之下，Zfpm-1功能增益与上述功能丧失结果相反。
Erythropoiesis is a complex multistage process for the differentiation of mature erythrocytes from hematopoietic stem cells. The function of several transcription factors has been reported in hematopoietic stem cell differentiation. However, the molecular basis governing its functional behavior is unclear. In this study, we characterized the role of Zfpm-1 during the erythropoietic differentiation of human hematopoietic stem cells. To verify the function of Zfpm-1 during erythropoietic differentiation, we established human CD34+cell culture system by using human umbilical cord blood. At day 7 of the human CD34+cell differentiation process to proerythocytes, Zfpm-1 was initially up-regulated and then dramatically down-regulated at day 9. The Zfpm-1 siRNA transfected HSCs contained 20% more GPA+cells than the mock transfected cells, and showed repressed expression of the hematopoietic transcription factors, c-myc and c-myb, but increased expression of GATA-1. In contrast, the Zfpm-1 gain-of-function is the opposite of loss-of-function results above.