ZESTE ENCODES A SEQUENCE-SPECIFIC TRANSCRIPTION FACTOR THAT ACTIVATES THE ULTRABITHORAX PROMOTER INVITRO
ZESTE ENCODES A SEQUENCE-SPECIFIC TRANSCRIPTION FACTOR THAT ACTIVATES THE ULTRABITHORAX PROMOTER INVITRO
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DOI：
10.1016/0092-8674(88)90089-x
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发表时间：
1988-06-03
期刊：
影响因子：
64.5
通讯作者：
TJIAN, R
中科院分区：
文献类型：
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作者：
BIGGIN, MD;BICKEL, S;TJIAN, R
Zeste是果蝇双胸复合体中的一个转录调控基因。在这里，我们发现纯化的zeste蛋白结合到多个位点，仅5“的Ubx RNA的起始位点。Zeste蛋白从果蝇细胞或E.表达zeste基因的大肠杆菌在体外激活Ubx转录。这种激活依赖于zeste蛋白结合位点的存在，因为在缺乏这些位点的Ubx启动子或Adh启动子的情况下没有观察到这种激活。 这些结果表明，transvection涉及的调控元件，在转录起始的水平上发挥作用，并可能是机械上类似的转录激活的增强子元件，除了transvection发生在配对的染色体。这些发现与Zeste可能在Ubx及其其他靶基因的正常调节中发挥比当前遗传证据所暗示的更重要的作用的假设一致。
Zeste is a Drosophila regulatory gene that is required for transvection at the bithorax complex. Here we find that purified zeste protein binds to multiple sites just 5'' of the initiation site of Ubx RNA. Zeste protein purified from Drosophila cells or from E. coli expressing the zeste gene activates Ubx transcription in vitro. This activation is dependent on the presence of zeste protein binding sites, as it is not observed with a Ubx promoter lacking these sites or with an Adh promoter. These results suggest that transvection involves regulatory elements that act at the level of transcriptional initiation and may be mechanistically similar to activation of transcription by enhancer elements, except that transvection occurs across paired chromosomes. These findings are consistent with the hypothesis that zeste may play a more important role in the normal regulation of Ubx and its other target genes than current genetic evidence implies.