A cDNA cloned from pregnant mouse uterus exhibits temporo-spatial expression and predicts a novel protein.
A cDNA cloned from pregnant mouse uterus exhibits temporo-spatial expression and predicts a novel protein.
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从怀孕小鼠子宫克隆的 cDNA 表现出时空表达并预测一种新蛋白质。
作者：
J. Kasik
从妊娠小鼠子宫cDNA文库中克隆了一个cDNA。在概念翻译中，cDNA有一个长的开放阅读框架，预测一个606个氨基酸的新蛋白质。该蛋白主要由两个CUB结构域和一个ZP结构域组成;在涉及卵子-精子识别的蛋白质中发现的基序。使用来自cDNA的探针进行北方杂交。这种mRNA的表达是暂时的;信息在出生前6天首次出现在子宫中，随后每天增加，在出生前3天达到最高水平，然后在怀孕的最后3天突然减少。这种mRNA的表达是有限的;信息是丰富的子宫在妊娠后期，但它是没有发现在非妊娠子宫或在各种成人或胎儿组织。这种怀孕子宫特异性mRNA的时空表达和与早期妊娠事件有关的两个基序的预测蛋白质的整合表明，这种mRNA所代表的基因的产物可能在妊娠晚期发生的事件中起重要作用。
A cDNA was cloned from a pregnant mouse uterus cDNA library. On conceptual translation, the cDNA has one long open reading frame that predicts a novel protein of 606 amino acids. This protein is principally composed of two CUB domains and a ZP domain; motifs found in proteins implicated in egg-sperm recognition. Probes derived from the cDNA were used to conduct Northern hybridizations. The expression of this mRNA is temporal; message first appears in the uterus 6 days prior to birth, it increases each subsequent day to attain maximal levels at 3 days prior to birth and then abruptly decreases during the last 3 days of pregnancy. The expression of this mRNA is restricted; message is abundant in the uterus during late pregnancy, but it is not found in non-pregnant uterus or in a variety of adult or fetal tissues. The temporo-spatial expression of this pregnant uterus specific mRNA and the consolidation in the predicted protein of two motifs implicated in early pregnancy events suggests that the product of the gene represented by this mRNA may play an important role in events that transpire during late pregnancy.