Nutrient Interactions and Toxicity Research Communication Safety Assessment of Recombinant Green Fluorescent Protein Orally Administered to Weaned Rats
Nutrient Interactions and Toxicity Research Communication Safety Assessment of Recombinant Green Fluorescent Protein Orally Administered to Weaned Rats
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断奶大鼠口服重组绿色荧光蛋白的营养相互作用和毒性研究通讯安全性评估
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
C. Stewart
中科院分区：
文献类型：
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作者：
H. Richards;Chung;R. Hopkins;M. Failla;W. Ward;C. Stewart
绿色荧光蛋白（GFP）的几个拟议的生物技术应用可能会导致其引入到家畜和人类的食品供应。我们喂养纯绿色荧光蛋白和饮食含有转基因油菜表达绿色荧光蛋白的年轻雄性大鼠26天，以评估潜在的毒性和过敏性的绿色荧光蛋白。动物（每组n 8）喂食AIN-93 G（对照）、对照饮食加每天1.0 mg纯化的GFP、具有200 g/kg卡诺拉（Brassica rapa cv Westar）的改良对照饮食或具有200 g/kg转基因卡诺拉的对照饮食，所述卡诺拉含有两种水平的GFP中的一种。摄入GFP不影响生长、食物摄入、肠或其他器官的相对重量或血清中肝酶的活性。将GFP的氨基酸序列与已知的食物过敏原进行比较，发现GFP与任何食物过敏原之间最多的连续氨基酸匹配是4个，这表明没有共同的过敏原表位。此外，GFP在模拟胃消化过程中迅速降解。这些数据表明，绿色荧光蛋白是一个低变应原性风险，并提供初步迹象表明，绿色荧光蛋白是不太可能代表一个健康风险。J.营养133：1909-1912，2003.
Several proposed biotechnological applications of green fluorescent protein (GFP) are likely to result in its introduction into the food supply of domestic animals and humans. We fed pure GFP and diets containing transgenic canola expressing GFP to young male rats for 26 d to evaluate the potential toxicity and allergenicity of GFP. Animals (n 8 per group) were fed either AIN-93G (control), control diet plus 1.0 mg of purified GFP daily, modified control diet with 200 g/kg canola (Brassica rapa cv Westar), or control diet with 200 g/kg transgenic canola containing one of two levels of GFP. Ingestion of GFP did not affect growth, food intake, relative weight of intestine or other organs, or activities of hepatic enzymes in serum. Comparison of the amino acid sequence of GFP to known food allergens revealed that the greatest number of consecutive amino acid matches between GFP and any food allergen was four, suggesting the absence of common allergen epitopes. Moreover, GFP was rapidly degraded during simulated gastric digestion. These data indicate that GFP is a low allergenicity risk and provide preliminary indications that GFP is not likely to represent a health risk. J. Nutr. 133: 1909–1912, 2003.