The Reverse Block Copolymer Pluronic 25R2 Promotes DNA Transfection of Skeletal Muscle
The Reverse Block Copolymer Pluronic 25R2 Promotes DNA Transfection of Skeletal Muscle
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DOI：
10.1002/mabi.201000463
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发表时间：
2011-05-12
影响因子：
4.6
通讯作者：
Kichler, Antoine
中科院分区：
文献类型：
--
作者：
Guiraud, Simon;Alimi-Guez, Debborah;Kichler, Antoine
肌肉是基因治疗的一个重要且有吸引力的目标。最近的研究结果表明，与裸露 DNA 相比，具有 PEO-PPO-PEO 排列的中性两亲性三嵌段共聚物显着增加肌肉转染。我们有兴趣评估反向 Pluronics (PPO-PEO-PPO) 是否也具有转染特性。因此，我们测量了 25R2 和 25R4 这两种亲水/疏水平衡不同的共聚物的体外和体内转染活性。结果表明，与裸露 DNA 相比，25R2 显着提高了肌肉中的转染水平。总而言之，这项工作表明反向 Pluronic 25R2 具有有趣的体内转染特性。
Muscle is an important and attractive target for gene therapy. Recent findings have shown that neutral amphiphilic triblock copolymers with a PEO-PPO-PEO arrangement significantly increase muscle transfection as compared to naked DNA. We were interested in evaluating whether reverse Pluronics (PPO-PEO-PPO) also possess transfection properties. Therefore, we measured the in vitro and in vivo transfection activity of 25R2 and 25R4, two copolymers that differ by their hydrophilic/hydrophobic balance. The results show that 25R2 significantly increases the transfection level in muscle compared to naked DNA. Taken together, this work demonstrates that the reverse Pluronic 25R2 possesses interesting properties for in vivo transfection.