Inhibition of Neisseria gonorrhoeae epithelial cell interactions by vaginal Lactobacillus species
Inhibition of Neisseria gonorrhoeae epithelial cell interactions by vaginal Lactobacillus species
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DOI：
10.1128/iai.00101-08
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发表时间：
2008-07-01
影响因子：
3.1
通讯作者：
Arvidson, Cindy Grove
中科院分区：
文献类型：
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作者：
Spurbeck, Rachel R.;Arvidson, Cindy Grove
高水平的乳酸杆菌是健康人类阴道微生物群的主要属，在流行病学上与暴露于性传播病原体淋病奈瑟菌后感染风险降低有关。在这项工作中，淋球菌感染的细胞培养模型，适用于检查乳酸杆菌淋球菌与子宫内膜上皮细胞在体外的相互作用的影响。用詹氏乳杆菌、加氏乳杆菌ATCC 33323或L.加氏ATCC 9857减少淋球菌粘附近50%。乳酸杆菌也抑制淋球菌入侵上皮细胞的60%以上，这是独立的粘附效果。此外，乳酸杆菌能够取代粘附淋球菌从上皮细胞，这表明这些生物体有潜力作为一个postexacetably预防。因此，阴道乳酸杆菌具有在感染的两个关键步骤抑制淋球菌的能力，这可能在确定淋球菌是否能够在体内暴露后成功建立感染方面具有显著作用。
High levels of Lactobacillus, the dominant genus of the healthy human vaginal microbiota, have been epidemiologically linked to a reduced risk of infection following exposure to the sexually transmitted pathogen Neisseria gonorrhoeae. In this work, a cell culture model of gonococcal infection was adapted to examine the effects of lactobacilli on gonococcal interactions with endometrial epithelial cells in vitro. Precolonization of epithelial cells with Lactobacillus jensenii, Lactobacillus gasseri ATCC 33323, or L. gasseri ATCC 9857 reduced gonococcal adherence by nearly 50%. Lactobacilli also inhibited gonococcal invasion of epithelial cells by more than 60%, which was independent of the effect on adherence. Furthermore, lactobacilli were able to displace adherent gonococci from epithelial cells, suggesting that these organisms have potential as a postexposure prophylactic. Thus, vaginal lactobacilli have the ability to inhibit gonococci at two key steps of an infection, which might have a significant effect in determining whether the gonococcus will be able to successfully establish an infection following exposure in vivo.