Indication of transcytotic movement of insulin across human bronchial epithelial cells.
Indication of transcytotic movement of insulin across human bronchial epithelial cells.
复制标题
胰岛素穿过人支气管上皮细胞的转胞吞运动的指示。
DOI：
10.1080/10611860600649633
复制
发表时间：
2006
影响因子：
4.5
通讯作者：
Ahsan,Fakhrul
中科院分区：
文献类型：
--
作者：
Hussain,Alamdar;Ahsan,Fakhrul
这项研究旨在评估胰岛素在人支气管上皮细胞系中的渗透性，并研究胰岛素是通过细胞旁途径还是跨细胞途径转运的。在辛基麦芽糖苷存在或不存在的情况下，研究了胰岛素在两种支气管上皮细胞16HBE14O-和CALU-3之间的迁移。甘露醇和心得安分别作为细胞旁和跨细胞标志物，并通过跨上皮电阻(TEER)检测单层紧密连接的完整性。用荧光标记的胰岛素孵育细胞后，用激光共聚焦扫描显微镜研究了胰岛素在这两种细胞系中可能的内吞机制。两个细胞单层的TEER值在汇合处为>；400Ωcm~2。在井间根尖侧加入辛基麦芽糖苷后，TEER值降低。同样，胰岛素、甘露醇和心得安的表观渗透系数(Papp)值也随着辛基麦芽糖苷浓度的升高而增大。在不含辛基麦芽糖苷的情况下，胰岛素及其标志物的Papp值顺序为：心得安+GT>甘露醇+GT>胰岛素。共聚焦显微镜研究表明，支气管上皮细胞对胰岛素的摄取可能是通过跨细胞转位进行的。这项研究提供的数据表明，胰岛素可能通过细胞旁和跨细胞途径跨支气管细胞移动。
This study was performed to evaluate insulin permeability across human bronchial epithelial cell lines and investigate if insulin is transported via the paracellular or transcellular pathway. The movement of insulin across two bronchial epithelial cells, 16HBE14o- and Calu-3, was studied in the presence or absence of octylmaltoside. Mannitol and propanolol have been used as paracellular and transcellular marker, respectively, and transepithelial electrical resistance (TEER) was determined to investigate the tight junctional integrity of the monolayers. The possible endocytotic mechanism of insulin across these two cell lines was studied by confocal laser scanning microscopy after incubating the cells with fluorescent-labeled insulin. The TEER values for both cell monolayers were >400 Ω cm2at confluency. There was a decrease in the TEER values when octylmaltoside was added to the apical side of transwells. Similarly, the apparent permeability coefficient (Papp) values of insulin, mannitol and propanolol, showed an increase with the rise in the concentration of octylmaltoside. In the absence of octylmaltoside, thePappvalues for insulin and the markers were in the following order: propanolol > mannitol > insulin. Confocal microscopic studies revealed that the uptake of insulin by the bronchial epithelial cells perhaps occurs via translocation across the cell. The data presented in this study demonstrate that insulin perhaps moves across the bronchial cells via both paracellular and transcellular pathways.