Selective Detection of Antigen-Antibody Reaction Using Si Ring Optical Resonators
Selective Detection of Antigen-Antibody Reaction Using Si Ring Optical Resonators
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DOI：
10.1143/jjap.49.04dl09
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发表时间：
2009-10
影响因子：
1.5
通讯作者：
Masataka Fukuyama;Shinya Yamatogi;H. Ding;Mizue Nishida;C. Kawamoto;Y. Amemiya;Takeshi Ikeda;T. Noda;S. Kawamoto;K. Ono;A. Kuroda;S. Yokoyama
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作者：
Masataka Fukuyama;Shinya Yamatogi;H. Ding;Mizue Nishida;C. Kawamoto;Y. Amemiya;Takeshi Ikeda;T. Noda;S. Kawamoto;K. Ono;A. Kuroda;S. Yokoyama
利用电子束光刻和反应离子刻蚀技术，制备了硅环光学谐振器。该谐振器用于感应受体-分析物反应，使用硅结合蛋白Si-tag，使受体固定在二氧化硅表面上，具有可控的取向。受体使用主要的屋尘螨过敏原derf2，分析物使用选择性结合derf2的抗derf2免疫球蛋白G型（IgG）。将含有抗Der f2 IgG的抗Der f2血清用纯水稀释到1/100，暴露在共振器上，Der f2被si标签吸附。然后将共振波长移动0.18 nm，而暴露于不含Der f2 IgG的正常兔血清中，峰值移动不到一半（0.07 nm）。这些结果表明Der f2与抗Der f2 IgG之间存在选择性反应。
Si ring optical resonators have been fabricated by the Si LSI technology using electron beam lithography and reactive ion etching. The resonator is applied for sensing receptor-analyte reaction using silicon-binding protein, Si-tag, which enables receptors immobilized on a Si dioxide surface with a controlled orientation. For the receptor, the major house-dust mite allergen called Der f 2 was used, and for the analyte anti-Der f 2 immunoglobulin type G (IgG), which is selectively bound to Der f 2, was used. Antiserum against Der f 2, which contains anti-Der f 2 IgG, was diluted to 1/100 with the pure water, and exposed to the resonator on which the Der f 2 was adsorbed with the Si-tag. Then the resonance wavelength was shifted by 0.18 nm, while exposure of the normal rabbit serum containing no Der f 2 IgG resulted in the peak shift less than half (0.07 nm). These results indicate the selective reaction between Der f 2 and anti-Der f 2 IgG.