A cell-laden microfluidic hydrogel
A cell-laden microfluidic hydrogel
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DOI：
10.1039/b615486g
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发表时间：
2007-06-01
期刊：
影响因子：
6.1
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
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作者：
Ling, Yibo;Rubin, Jamie;Khademhosseini, Ali
将哺乳动物细胞封装在微流体通道的主体材料内对于从组织工程到基于细胞的诊断测定的应用范围可能是有益的。在这项工作中，我们提出了一种技术，用于制造微流体通道从细胞负载琼脂糖水凝胶。使用标准的软平版印刷技术，将熔融琼脂糖对着SU-8图案化的硅晶片模制。为了产生密封的和水密的微流体通道，将模制琼脂糖的表面在71 ° C下加热3秒，并密封到另一个表面加热的琼脂糖板。产生了不同尺寸的通道，结果表明，琼脂糖，虽然高度多孔，是一个合适的材料进行微流体。嵌入微流体模具中的细胞分布均匀，通过通道泵送的培养基允许营养物质和废物的交换。虽然发现大多数细胞在初始装置制造时是活的，但只有靠近微流体通道的那些细胞在3天后仍然是活的，这证明了微通道的灌注网络对于递送营养物和氧气以维持大水凝胶中的细胞活力的重要性。该技术的进一步发展可能导致仿生合成血管的产生，用于组织工程、诊断和药物筛选应用。
The encapsulation of mammalian cells within the bulk material of microfluidic channels may be beneficial for applications ranging from tissue engineering to cell-based diagnostic assays. In this work, we present a technique for fabricating microfluidic channels from cell-laden agarose hydrogels. Using standard soft lithographic techniques, molten agarose was molded against a SU-8 patterned silicon wafer. To generate sealed and water-tight microfluidic channels, the surface of the molded agarose was heated at 71 degrees C for 3 s and sealed to another surface-heated slab of agarose. Channels of different dimensions were generated and it was shown that agarose, though highly porous, is a suitable material for performing microfluidics. Cells embedded within the microfluidic molds were well distributed and media pumped through the channels allowed the exchange of nutrients and waste products. While most cells were found to be viable upon initial device fabrication, only those cells near the microfluidic channels remained viable after 3 days, demonstrating the importance of a perfused network of microchannels for delivering nutrients and oxygen to maintain cell viability in large hydrogels. Further development of this technique may lead to the generation of biomimetic synthetic vasculature for tissue engineering, diagnostics, and drug screening applications.