Mapping of quantitative trait loci for canopy-wilting trait in soybean (Glycine max L. Merr)
Mapping of quantitative trait loci for canopy-wilting trait in soybean (Glycine max L. Merr)
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DOI：
10.1007/s00122-012-1876-9
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发表时间：
2012-09-01
影响因子：
5.4
通讯作者：
Boerma, H. Roger
中科院分区：
文献类型：
--
作者：
Abdel-Haleem, Hussein;Carter, Thomas E., Jr.;Boerma, H. Roger
干旱胁迫对大豆（Glycine max（L.）在大多数发育阶段，这共同导致产量降低。关于大豆耐旱性数量性状基因座（QTL）的相对贡献率和染色体定位的信息很少。发现日本种质资源PI 416937具有抗旱性。在水分亏缺条件下，PI 416937在田间枯萎比优良品种更慢，并已被用作育种计划中的亲本以提高大豆产量。从PI 416937和Benning之间的杂交获得重组自交系（RIL）群体，并且在五种不同环境中对群体在雨养田间条件下的冠层萎蔫进行表型分析，以鉴定与冠层萎蔫性状相关的QTL。在环境组合分析中，在不同的染色体上发现了7个QTL，它们解释了75%的冠层萎蔫性状变异，这表明该性状的复杂性。5个QTL从PI 416937中遗传了各自的正等位基因。令人惊讶的是，另外两个QTL从Benning遗传了它们的正等位基因。这些推定的QTL共定位与其他QTL先前确定为相关的植物非生物胁迫在大豆中，表明冠层萎蔫QTL可能与其他形态生理性状在大豆。在组合分析中以及在每个单独的环境中检测到来自PI 416937的染色体12（Gm12）上的基因座，并且解释了27%的冠层萎蔫变异。在PI 416937中发现的QTL可以为大豆耐旱品种的田间培育提供一种有效的手段。
Drought stress adversely affects [Glycine max (L.) Merr] soybean at most developmental stages, which collectively results in yield reduction. Little information is available on relative contribution and chromosomal locations of quantitative trait loci (QTL) conditioning drought tolerance in soybean. A Japanese germplasm accession, PI 416937, was found to possess drought resistance. Under moisture-deficit conditions, PI 416937 wilted more slowly in the field than elite cultivars and has been used as a parent in breeding programs to improve soybean productivity. A recombinant inbred line (RIL) population was derived from a cross between PI 416937 and Benning, and the population was phenotyped for canopy wilting under rain-fed field conditions in five distinct environments to identify the QTL associated with the canopy-wilting trait. In a combined analysis over environments, seven QTL that explained 75 % of the variation in canopy-wilting trait were identified on different chromosomes, implying the complexity of this trait. Five QTL inherited their positive alleles from PI 416937. Surprisingly, the other two QTL inherited their positive alleles from Benning. These putative QTL were co-localized with other QTL previously identified as related to plant abiotic stresses in soybean, suggesting that canopy-wilting QTL may be associated with additional morpho-physiological traits in soybean. A locus on chromosome 12 (Gm12) from PI 416937 was detected in the combined analysis as well as in each individual environment, and explained 27 % of the variation in canopy-wilting. QTL identified in PI 416937 could provide an efficient means to augment field-oriented development of drought-tolerant soybean cultivars.