Glutamatergic Kolliker-Fuse nucleus neurons innervate hypoglossal motoneurons whose axons form the medial (protruder)branch of the hypoglossal nerve in therat
Glutamatergic Kolliker-Fuse nucleus neurons innervate hypoglossal motoneurons whose axons form the medial (protruder)branch of the hypoglossal nerve in therat
复制标题
谷氨酸科利克融合核神经元支配舌下运动神经元，其轴突形成大鼠舌下神经的内侧（突出）分支
DOI：
10.1016/j.brainres.2011.06.025
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发表时间：
2011
期刊：
影响因子：
2.9
通讯作者：
Oka T,Yasui Y
中科院分区：
文献类型：
--
作者：
Yokota S;Niu JG;Tsumori T;Oka T,Yasui Y
本研究旨在了解Kölliker-Fuse核（KFN）调节呼吸相关舌运动的解剖学基础。将霍乱毒素B亚基（CTb）应用于舌下神经（HGn）内侧支，并在舌下神经同侧注射生物素化右旋糖酐胺（BDA）后，发现BDA标记的轴突末梢与CTb标记的神经元在舌下核（HGn）同侧的腹侧腔室重叠分布。在电镜下，bda标记的末端主要与HGN神经元的树突进行不对称的突触接触，其中部分树突被CTb标记。通过逆行示踪结合原位杂交，我们发现几乎所有向HGN发送轴突的KFN神经元的vesicular glutamate transporter (VGLUT) 2 mRNA均呈阳性，但谷氨酸脱羧酶67 mRNA呈阴性。利用VGLUT2的顺、逆行示踪技术和免疫组化相结合，我们进一步证明了具有VGLUT2免疫反应性的KFN轴突末端与HGN运动神经元建立了密切联系，HGN运动神经元的轴突构成了HGN的内侧分支。本研究结果提示，谷氨酸能KFN纤维可能对HGN运动神经元发挥兴奋作用，将轴突发送到HGN内侧分支，控制舌突肌收缩，维持呼吸时气道通畅。
This study was performed to understand the anatomical substrates for Kölliker–Fuse nucleus (KFN) modulation of respiratory-related tongue movement. After application of cholera toxin B subunit (CTb) to the medial branch of the hypoglossal nerve (HGn) and injection of biotinylated dextran amine (BDA) into the KFN ipsilaterally, an overlapping distribution of BDA-labeled axon terminals and CTb-labeled neurons was found in the ventral compartment of the hypoglossal nucleus (HGN) ipsilateral to the application and injection sites. At the electron microscopic level, the BDA-labeled terminals made asymmetrical synaptic contacts predominantly with dendrites of the HGN neurons, some of which were labeled with CTb. Using retrograde tracing combined with in situ hybridization, we demonstrated that almost all the KFN neurons sending their axons to the HGN were positive for vesicular glutamate transporter (VGLUT) 2 mRNA but not glutamic acid decarboxylase 67 mRNA. Using a combination of anterograde and retrograde tracing techniques and immunohistochemistry for VGLUT2, we further demonstrated that the KFN axon terminals with VGLUT2 immunoreactivity established close contact with the HGN motoneurons whose axons constitute the medial branch of the HGn. The present results suggest that glutamatergic KFN fibers may exert excitatory influence upon the HGN motoneurons sending their axons to the medial branch of the HGn for the control of protruder tongue muscles contraction to maintain airway patency during respiration.