Regionalized cadherin-7 expression by radial glia is regulated by Shh and Pax7 during chicken spinal cord development
Regionalized cadherin-7 expression by radial glia is regulated by Shh and Pax7 during chicken spinal cord development
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DOI：
10.1016/j.neuroscience.2006.07.038
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发表时间：
2006-11-03
期刊：
影响因子：
3.3
通讯作者：
Redies, C.
中科院分区：
文献类型：
--
作者：
Luo, J.;Ju, M. J.;Redies, C.
在发育过程中，一些指定神经元亚型身份的基因在脊髓和后脑不同的背腹侧区域表达。cadherin -7 （Cad7）是粘着分子cadherin家族的一员，在鸡脊髓基底背区由径向胶质细胞表达。为了研究Cad7基因的调控，我们通过体内电穿孔在不同发育阶段的尾侧神经管和两个脑区异位表达了两个已知的背腹侧模式基因Shh和Pax7。结果表明，Shh以浓度依赖性的方式调节径向胶质细胞中Cad7的表达。在低浓度和高浓度下，Shh分别诱导或抑制Cad7的表达。此外，Pax7抑制Cad7的表达。这些结果与Shh和Pax7在脊髓和后脑发育过程中调节内源性Cad7表达的作用一致。我们的数据首次表明，Shh不仅可以调节其他基因调控因子的表达，还可以调节Cad7的表达，Cad7是一种在轴突延伸和神经回路形成中起作用的形态调控分子。(c) 2006 IBRO。Elsevier Ltd.出版。版权所有。
During development, several genes that specify neuronal subtype identity are expressed in distinct dorsoventral domains of the spinal cord and hindbrain. Cadherin-7 (Cad7), a member of the cadherin family of adhesion molecules, is expressed by radial glia in a dorsal domain of the spinal cord basal plate in chicken. To study the regulation of the Cad7 gene, we ectopically expressed two known dorsoventral patterning genes, Shh and Pax7, in the caudal neural tube and in two brain regions at different stages of development by in vivo electroporation. Results showed that Shh regulated the expression of Cad7 by radial glia in a concentration-dependent manner. Shh induced or repressed the expression of Cad7, at low and high concentrations, respectively. Furthermore, Pax7 inhibited the expression of Cad7. These results are compatible with a role of Shh and Pax7 in regulating endogenous Cad7 expression during spinal cord and hindbrain development. Our data show, for the first time, that Shh can regulate the expression not only of other gene regulatory factors, but also of Cad7, a morphoregulatory molecule that plays a role in axon elongation and neural circuit formation. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.