Assembly of nucleosomal templates by salt dialysis.
Assembly of nucleosomal templates by salt dialysis.
复制标题
通过盐透析组装核小体模板。
DOI：
10.1002/0471142727.mb2106s54
复制
发表时间：
2001
影响因子：
--
通讯作者：
G. Narlikar
中科院分区：
文献类型：
--
作者：
K. M. Lee;G. Narlikar
由于通过盐渗析组装核小体模板很方便，因此可以在很短的时间内容易地制备出大量的染色质复合体。本单元包括各种盐透析方案(分级和梯度)的说明，并附有从细菌中提纯核心组蛋白和为核小体阵列准备DNA的方案。还包括分析重组络合物的电泳法。
Because it is convenient to assemble nucleosomal templates through salt dialysis, large amounts of chromatin complexes can be made easily and in a short amount of time. This unit includes instructions for the various salt dialysis schemes (step versus gradient), which are accompanied by protocols for purification of core histones from bacteria and preparation of DNA for the nucleosomal arrays. Electrophoretic procedures to analyze the reconstituted complexes are also included.