Homogeneity of Streptococcus dysgalactiae from farmed amberjack Seriola dumerili in Japan
Homogeneity of Streptococcus dysgalactiae from farmed amberjack Seriola dumerili in Japan
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DOI：
10.1007/s12562-010-0243-5
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发表时间：
2010-07-01
影响因子：
1.9
通讯作者：
Yoshida, Terutoyo
中科院分区：
文献类型：
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作者：
Nishiki, Issei;Noda, Masahiro;Yoshida, Terutoyo
停乳链球菌菌株已从日本养殖的鰤鰤和黄尾鰤鱼中分离出来。为了表征鱼类分离株，我们进行了遗传分析，并将这些分离株与停乳链球菌亚种的生化特性进行了比较。停乳症和停乳链球菌亚种。从哺乳动物中分离出的似马菌株。遗传分析表明，这些鱼在遗传上非常相似，具有较高的DNA-DNA相关性（>95.4%）和序列同源性。同时，哺乳动物分离株与鱼类分离株之间的DNA相关性为73.4-82.6%。在偏正弦凝胶电泳 (BSFGE) 分析中，哺乳动物分离株的限制模式与鱼类分离株不同。鱼类分离物在从哺乳动物获得的纤溶酶原中不显示链激酶活性。这些特征使我们能够区分鱼类分离株和从哺乳动物中分离出的 Sdd 和 Sde 菌株。为了获得鱼类分离株的流行病学信息，对日本鱼类中 284 个停乳链球菌菌株的 BSFGE 模式进行了检查。根据BSFGE分析结果，用限制性内切酶ApaI将鱼分离物分为16组（AP1-AP16）。基于 BSFGE 分析的树状图表明本研究中使用的所有鱼类分离株密切相关。
Streptococcus dysgalactiae strains have been isolated from cultured amberjack Seriola dumerili and yellowtail Seriola quinqueradiata in Japan. To characterize the fish isolates, we performed genetic analysis and compared the biochemical properties of these isolates with those of the S. dysgalactiae subsp. dysgalactiae and S. dysgalactiae subsp. equisimilis strains isolated from mammals. The genetic analysis revealed that the fish isolates were genetically very similar to each other with high DNA-DNA relatedness (>95.4%) and sequence homology. Meanwhile, the DNA relatedness between mammalian isolates and the fish isolates was 73.4-82.6%. In biased sinusoidal gel electrophoresis (BSFGE) analysis, the restriction patterns of mammalian isolates were different from those of fish isolates. The fish isolates did not show streptokinase activity in plasminogen obtained from mammals. These characteristics enabled us to distinguish between the fish isolates and the Sdd and Sde strains isolated from mammals. In order to obtain epidemiological information on the fish isolates, BSFGE patterns from 284 S. dysgalactiae strains from fish in Japan were examined. Based on the results of BSFGE analysis, the fish isolates were classified into 16 groups (AP1-AP16) with restriction enzyme ApaI. The dendrogram based on BSFGE analysis indicated that all fish isolates using in this study were closely related.