The relationship between microbiota and polyamine concentration in the human intestine: A pilot study
The relationship between microbiota and polyamine concentration in the human intestine: A pilot study
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DOI：
10.1111/j.1348-0421.2007.tb03887.x
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发表时间：
2007-01-01
影响因子：
2.6
通讯作者：
Benno, Yoshimi
中科院分区：
文献类型：
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作者：
Matsumoto, Mitsuharu;Benno, Yoshimi
采用末端限制性片段长度多态性（T-RFLP）分析方法，对10名住院老年人和14名健康成人的粪便微生物群进行了HhaI、MspI、HaeIII和MspI分析，并测定了粪便多胺（PA）浓度。受试者粪便微生物群的T-RFLP谱大致分为2个聚类-I（11个中的9个来自住院的老年受试者）和II（13个中的12个来自健康成人）。集群II中腐胺的平均浓度是集群I中腐胺的5.8倍（P=0.0015）。使用用于人类结肠微生物群的T-RFLP分析的系统发育分配数据库，在具有高粪便PA浓度的受试者的情况下特征性地检测到的末端限制性片段（T-RF）被预测为源自属于梭菌亚群XIVa的细菌物种和细菌类型，特别是包括解木聚糖梭菌、解糖梭菌、未培养的人肠道细菌克隆JW 1H 4（Desulfotomaculum guttoideum的近缘种）、罗斯拜瑞氏菌（Roseburia）、未培养的细菌克隆41 F 10（Eubacterium ramulus的近缘种）、盲肠罗斯拜瑞氏菌（Roseburia cecicola）、粗瘤胃球菌（Ruminococcus obeum）及其近缘种。从这些结果中，我们得出结论，粪便微生物群可能与粪便PA浓度有关，并且属于梭菌亚群XIVa的一些细菌物种可能在控制人类肠道PA浓度中发挥重要作用。
The fecal microbiota of 10 hospitalized elderly subjects and 14 healthy adults were analyzed by terminal-restriction fragment length polymorphism (T-RFLP) analysis using HhaI, MspI, HaeIII, and AluI, as well as fecal polyamine (PA) concentration. The T-RFLP profiles of the fecal microbiota of the subjects were roughly divided into 2 clusters-I (9 out of 11 were derived from hospitalized elderly subjects) and II (12 out of 13 were derived from healthy adults). The average concentration of putrescine in Cluster II was 5.8 times higher than that of putrescine in Cluster I (P=0.0015). Using a phylogenetic assignment database for T-RFLP analysis of human colonic microbiota, the terminal-restriction fragments (T-RFs) characteristically detected in the case of subjects with high fecal PA concentration were predicted to be derived from bacterial species and phylotypes belonging to Clostridium subcluster XIVa, particularly including Clostridium xylanolyticum, Clostridium saccharolyticum, the uncultured human intestinal bacterium clone JW1H4 (a relative of Desulfotomaculum guttoideum), Roseburia intestinalis, the uncultured bacterium clone 41F10 (a relative of Eubacterium ramulus), Roseburia cecicola, Ruminococcus obeum and its relatives. From these results, we concluded that fecal microbiota may be linked with fecal PA concentration and that some bacterial species belonging to Clostridium subcluster XIVa may play a major role in the control of intestinal PA concentration in humans.