Current practices in antinuclear antibody testing: results from the Belgian External Quality Assessment Scheme
Current practices in antinuclear antibody testing: results from the Belgian External Quality Assessment Scheme
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DOI：
10.1515/cclm.2009.021
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发表时间：
2009-01-01
影响因子：
6.8
通讯作者：
Libeer, Jean-Claude
中科院分区：
文献类型：
--
作者：
Van Blerk, Marjan;Van Campenhout, Christel;Libeer, Jean-Claude
背景资料：本研究利用2000 ~ 2005年比利时国家室间质量评价计划（NQA）的结果，对比利时和卢森堡实验室抗核抗体（ANA）检测的现状进行评价。参与者被调查的方法，并要求报告染色模式和ANA滴度。在2002年，试图提高定量ANA结果的可比性提供了一个商业参考材料，并与观察到的差异methodology.Results：除一个例外，所有参与者采用了显微镜为基础的间接免疫荧光检测与人类上皮细胞系2细胞。大多数实验室在描述这种模式时是准确的。对于抗体水平处于临界水平的样本和显示细胞质模式的样本，不可接受答案的百分比更大。观察到抗着丝粒抗体检测的改进。对于所有样品，报告了广泛的滴度范围。提供二级参比制剂可改善实验室间一致性。方法学变量的比较揭示了非标准化滴度与显微镜灯功率和暗室使用之间的相关性。本工作中呈现的EQAS结果为比利时和卢森堡实验室实践的ANA检测的最新技术水平提供了有价值的见解，并说明了ANA检测的国家EQAS作为提高性能和实验室间结果的可比性。
Background: This study aimed to assess the state-of-the-art of antinuclear antibody (ANA) testing as practiced in the Belgian and Luxembourg laboratories, using the results obtained in the Belgian National External Quality Assessment Scheme from 2000 to 2005.Methods: During this period, nine samples with different specificities were sent for analysis. Participants were surveyed for methodology used and were asked to report staining pattern and titer of ANAs. In 2002, an attempt was made to improve the comparability of quantitative ANA results by the provision of a commercial reference material and to relate observed differences to methodology.Results: With one exception, all participants employed a microscope-based indirect immunofluorescence assay with human epithelial cell line 2 cells. Most laboratories were accurate in describing the pattern. The percentage of unacceptable answers was greater for samples with borderline levels of antibody and for samples showing a cytoplasmic pattern. An improvement in the detection of anticentromere antibodies was observed. For all samples, a wide range of titers was reported. The provision of the secondary reference preparation led to improved inter-laboratory concordance. Comparison of methodology variables revealed a correlation between unstandardized titers and the power of the lamp of the microscope and the use of a dark room.Conclusions: The EQAS results presented in this work provide valuable insights into the state of the art of ANA testing as practiced in the Belgian and Luxembourg Laboratories and illustrate the important value of a national EQAS for ANA testing as a tool to improve performance and interlaboratory comparability of laboratory results.