P2X(3) Receptor in Primary Afferent Neurons Mediates the Relief of Visceral Hypersensitivity by Electroacupuncture in an Irritable Bowel Syndrome Rat Model.
P2X(3) Receptor in Primary Afferent Neurons Mediates the Relief of Visceral Hypersensitivity by Electroacupuncture in an Irritable Bowel Syndrome Rat Model.
复制标题
初级传入神经元中的 P2X3 受体通过电针介导肠易激综合征大鼠模型内脏过敏的缓解
DOI：
10.1155/2020/8186106
复制
发表时间：
2020
影响因子：
2
通讯作者：
Wu H
中科院分区：
文献类型：
--
作者：
Zhang F;Ma Z;Weng Z;Zhao M;Zheng H;Wu L;Lu Y;Bao C;Liu Y;Liu H;Wu H
研究背景电针治疗肠易激综合征（IBS）已被证实有效，外周和中枢神经元P2 X3受体参与电针介导的IBS内脏痛缓解。目的探讨腰骶段背根节结肠初级感觉神经元P2 X3受体参与电针减轻肠易激综合征（IBS）大鼠内脏高敏感性的神经生物学机制。方法参照Al-Chaer等方法建立IBS慢性内脏痛大鼠模型，电针双侧合母穴ST 25、ST 37进行干预。采用行为学、结肠组织病理学、电生理学、免疫荧光组织化学和实时荧光聚合酶链反应等方法，观察结肠P2 X3受体及相关背根神经节在电针减轻内脏高敏感性中的作用。结果电针可显著降低IBS大鼠在不同水平（20、40、60、80 mmHg）结肠扩张刺激下的行为学评分，下调IBS大鼠结肠及相关背根神经节P2 X3受体蛋白和mRNA的表达水平。电针还可调节IBS大鼠背根节结肠相关神经元的静息膜电位、基强度和动作电位等电特性。结论电针可调节IBS内脏痛大鼠结肠及相关DRG P2 X3受体蛋白和mRNA的表达水平，进而调节DRG神经元的兴奋特性。
Background Electroacupuncture (EA) has been confirmed effectiveness in the treatment of irritable bowel syndrome (IBS), and P2X3 receptors in the peripheral and central neurons participate in the acupuncture-mediated relief of the visceral pain in IBS. Objective To reveal the neurobiological mechanism that P2X3 receptor of colonic primary sensory neurons in the dorsal root ganglia of the lumbosacral segment is involved in the alleviation of visceral hypersensitivity by EA in an IBS rat model. Methods The IBS chronic visceral pain rat model was established according to the method of Al-Chaer et al. EA at the bilateral He-Mu points, including ST25 and ST37, was conducted for intervention. The behavioral studies, histopathology of colon, electrophysiology, immunofluorescence histochemistry, and real-time polymerase chain reaction assays were used to observe the role of P2X3 receptor in the colon and related DRG in relieving visceral hypersensitivity by EA. Results EA significantly reduced the behavior scores of the IBS rats under different levels (20, 40, 60, 80 mmHg) of colorectal distention stimulation and downregulated the expression levels of P2X3 receptor protein and mRNA in colon and related DRG of the IBS rats. EA also regulated the electrical properties of the membranes, including the resting membrane potential, rheobase, and action potential of colon-associated DRG neurons in the IBS rats. Conclusion EA can regulate the P2X3 receptor protein and mRNA expression levels in the colon and related DRG of IBS rats with visceral pain and then regulate the excitatory properties of DRG neurons.
登录
查看更多内容
影响因子：
24.5
作者：
Tillisch, K
通讯作者：
Tillisch, K
DOI：
10.1073/pnas.252537299
发表时间：
2002-12-24
影响因子：
11.1
作者：
Jarvis, MF;Burgard, EC;Faltynek, C
通讯作者：
Faltynek, C
DOI：
10.5114/pg.2018.78343
发表时间：
2018-01-01
影响因子：
1.3
作者：
Pietrzak, Anna;Skrzydlo-Radomanska, Barbara;Rydzewska, Grazyna
通讯作者：
Rydzewska, Grazyna
产前母体应激通过激活初级感觉神经元中的胱硫醚-β-合酶信号诱导成年大鼠后代的内脏超敏反应。
影响因子：
3.3
作者：
Wang HJ;Xu X;Xie RH;Rui YY;Zhang PA;Zhu XJ;Xu GY
通讯作者：
Xu GY
DOI：
10.1152/ajpgi.00257.2012
发表时间：
2012-10-01
影响因子：
4.5
作者：
Feng, Bin;La, Jun-Ho;Gebhart, G. F.
通讯作者：
Gebhart, G. F.