CLONING AND CHARACTERIZATION OF A DROSOPHILA TYRAMINE RECEPTOR
CLONING AND CHARACTERIZATION OF A DROSOPHILA TYRAMINE RECEPTOR
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DOI：
10.1002/j.1460-2075.1990.tb07572.x
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发表时间：
1990-11-01
期刊：
影响因子：
11.4
通讯作者：
HEN, R
中科院分区：
文献类型：
--
作者：
SAUDOU, F;AMLAIKY, N;HEN, R
生物胺受体，如多巴胺、血清素和肾上腺素，属于与G蛋白相互作用的受体家族，并共享假定的七个跨膜结构域结构。使用基于相应基因之间核苷酸序列同源性的策略，我们分离出编码G蛋白偶联受体家族新成员的果蝇cDNA克隆。该蛋白与人类α具有最高的同源性。2肾上腺素能受体，人类5HT1A受体和最近克隆的果蝇血清素受体。相应的mRNA主要存在于成年果蝇的头部。来自表达该受体的哺乳动物细胞的膜显示出对[3H]ohimbine和α的高亲和力结合位点。2-肾上腺素能受体拮抗剂（Kd = 4.45）。9米)。酪胺是果蝇神经递质中取代[3H]育亨宾结合效率最高的(EC50 = 1.25。10 - 6米)。此外，酪胺诱导表达该受体的NIH 3T3细胞抑制腺苷酸环化酶活性。因此，我们分离出的果蝇酪胺受体可能是一种无脊椎动物，相当于哺乳动物的α。2种肾上腺素能受体。
Receptors for biogenic amines such as dopamine, serotonin and epinephrine belong to the family of receptors that interact with G proteins and share a putative seven transmembrane domain structure. Using a strategy based on nucleotide sequence homology between the corresponding genes, we have isolated Drosophila cDNA clones encoding a new member of the G protein-coupled receptor family. This protein exhibits highest homology to the human .alpha.2 adrenergic receptors, the human 5HT1A receptor and a recently cloned Drosophila serotonin receptor. The corresponding mRNA is found predominantly in adult Drosophila heads. Membranes from mammalian cells expressing this receptor displayed high affinity binding sites for [3H]ohimbine, an .alpha.2-adrenergic receptor antagonist (Kd = 4.45 .times. 10-9 M). Tyramine was the most efficient of the putative Drosophila neurotransmitters at displacing [3H]yohimbine binding (EC50 = 1.25 .times. 10-6 M). Furthermore tyramine induced an inhibition of adenylate cyclase activity in NIH 3T3 cells expressing this receptor. The Drosophila tyramine receptor that we have isolated might therefore be an invertebrate equivalent of the mammalian .alpha.2 adrenergic receptors.