ELASTIN DEGRADATION IN ABDOMINAL AORTIC-ANEURYSMS
ELASTIN DEGRADATION IN ABDOMINAL AORTIC-ANEURYSMS
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DOI：
10.1016/0021-9150(87)90003-7
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发表时间：
1987-05-01
期刊：
影响因子：
5.3
通讯作者：
POWELL, JT
中科院分区：
文献类型：
--
作者：
CAMPA, JS;GREENHALGH, RM;POWELL, JT
腹主动脉瘤壁的组织学切片显示稀少且断裂的弹性组织。腹主动脉中膜的弹性蛋白含量仅为8.1±-。3.2%干脱脂重量(n=11)。大体正常年龄和解剖匹配的主动脉中膜的弹性蛋白含量为35.0±-。3.2%干重(n=4)，严重动脉粥样硬化、狭窄的肾下主动脉中层弹性蛋白含量为22.0±-。7.2%干重(n=6)。弹性蛋白含量与主动脉中膜匀浆的弹性蛋白溶解活性呈负相关，r=-0.78。弹性酶的活性，通过[~3H]弹性蛋白的水解度来衡量，在动脉瘤的主动脉匀浆中最高，为92.1±-。43.7U/mg蛋白质(n=18)，降至46.9+-。13.3U/mg蛋白(n=13)在严重狭窄的动脉粥样硬化性主动脉匀浆和35.5+-。大体正常主动脉匀浆中11.9U/mg(n=6)。狭窄主动脉的弹性蛋白溶解活性以白细胞弹性蛋白酶为重要成分。在动脉瘤匀浆中也发现了白细胞弹性蛋白酶，但弹性酶活性的增加是由于在2M尿素中提取的一种蛋白酶(S)(Mr 95000)，对丙氨酸键的特异性很低，并且与白细胞弹性蛋白酶没有免疫交叉反应。
Histological sections through the walls of abdominal aortic aneurysms showed scarce and disrupted elastic tissue. The elastin content of the aneurysmal aortic media was only 8.1 .+-. 3.2% dry defatted weight (n = 11). The elastin content of grossly normal age and anatomically matched aortic media was 35.0 .+-. 3.2% dry weight (n = 4) and the elastin content of severely atherosclerotic, stenosed infrarenal aortic media was 22.0 .+-. 7.2% dry weight (n =6). There was an inverse correlation of elastin content with the elastinolytic activity of aortic media homogenates, r = -0.78. Elastase activity, measured by the hydrolysis of [3H]elastin, was highest in aneurysmal aortic homogenates, 92.1 .+-. 43.7 U/mg protein (n = 18), falling to 46.9 .+-. 13.3 U/mg protein (n = 13) in severely stenosed atherosclerotic aortic homogenates and 35.5 .+-. 11.9 U/mg (n = 6) in grossly normal aortic homogenates. The elastinolytic activity of stenotic aorta contained leukocyte elastase as an important component. In aneurysmal homogenates leukocyte elastase was also found but the increased elastase activity resulted from a protease(s) (Mr 95,000) extracted in 2 M urea, having minimal specifity for alanyl bonds and no immunological cross-reactivity with leukocyte elastase.