Comparative expressed sequence hybridization reveals differential gene expression in morphological breast cancer subtypes
Comparative expressed sequence hybridization reveals differential gene expression in morphological breast cancer subtypes
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DOI：
10.1002/path.1911
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发表时间：
2006-03-01
影响因子：
7.3
通讯作者：
De Wolf-Peeters, C
中科院分区：
文献类型：
--
作者：
Bempt, IV;Vanhentenrijk, V;De Wolf-Peeters, C
在这项研究中，比较表达序列杂交（CESH）已被用于比较基因表达模式在三种形态不同的乳腺癌亚型：经典型浸润性小叶癌（ILC），低分化ERBB 2阴性浸润性导管癌-未另行说明（IDC-NOS），和低分化ERBB 2阳性IDC-NOS。CESH允许以类似于比较基因组杂交（CGH）的方式全局检测具有差异基因表达的染色体区域。每个乳腺癌亚型的8个病例被纳入研究。对于每种亚型，构建两个样本库，每个样本库有四个病例。使用CESH比较相同形态学亚型内的两个样本池，然后比较属于不同亚型的样本池。这揭示了三个染色体区域在导管癌和小叶癌中差异表达，包括8 q13-q23和16 q22的相对过表达，以及8 p21-p22的相对低表达。此外，ERBB 2阳性IDC-NOS的表达特征为3q 24-q26.3、14 q23 -31、17 q12和20 q12 -13的相对过表达。总之，CESH分析突出了与形态学上定义的乳腺癌亚型相关的差异基因表达的染色体区域，并表明8号染色体上的区域在导管癌和小叶癌之间的区分中具有重要意义。此外，使用CESH，可以鉴定ERBB 2表达特征，其包括在ERBB 2阳性IDC-NOS的侵袭行为中具有潜在意义的四个染色体区域。版权所有（c）2006大不列颠和爱尔兰病理学会。出版社：John Wiley & Sons，Ltd
In this study, comparative expressed sequence hybridization (CESH) has been used to compare gene expression patterns in three morphologically different breast cancer subtypes: classic-type invasive lobular carcinoma (ILC), poorly differentiated ERBB2-negative invasive ductal carcinoma-not otherwise specified (IDC-NOS), and poorly differentiated ERBB2-positive IDC-NOS. CESH allows global detection of chromosomal regions with differential gene expression in a way similar to that of comparative genomic hybridization (CGH). Eight cases of each breast cancer subtype were included in the study. For each subtype, two pools of four cases each were constructed. CESH was used to compare both pools within the same morphological subtype, followed by a comparison of pools belonging to different subtypes. This revealed three chromosomal regions that were differentially expressed in ductal and lobular carcinomas, including relative overexpression at 8q13-q23 and 16q22, and relative underexpression at 8p21-p22. In addition, an expression signature characterized by relative overexpression at 3q24-q26.3, 14q23-31, 17q12, and 20q12-13 was identified for ERBB2-positive IDC-NOS. In summary, CESH analysis highlights chromosomal regions of differential gene expression that are associated with morphologically defined breast cancer subtypes and suggests that regions on chromosome 8 are of interest in the discrimination between ductal and lobular carcinomas. In addition, using CESH, it was possible to identify an ERBB2 expression signature, comprising four chromosomal regions with potential significance in the aggressive behaviour of ERBB2-positive IDC-NOS. Copyright (c) 2006 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.