Endogenous production of n-3 long-chain PUFA from first feeding and the influence of dietary linoleic acid and the a-linolenic:linoleic ratio in Atlantic salmon (Salmo salar).
Endogenous production of n-3 long-chain PUFA from first feeding and the influence of dietary linoleic acid and the a-linolenic:linoleic ratio in Atlantic salmon (Salmo salar).
复制标题
大西洋鲑鱼 (Salmo salar) 首次饲喂时 n-3 长链 PUFA 的内源产生以及日粮亚油酸和 a-亚麻酸：亚油酸比例的影响。
DOI：
10.1017/s0007114519001946
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Sprague M
中科院分区：
文献类型：
--
作者：
Sprague M
大西洋鲑鱼(Salmo Salar)具有从α-亚麻酸(ALA)内源生物合成n-3长链多不饱和脂肪酸(LC-PUFA)所需的酶。亚油酸(LA)和丙氨酸(ALA)竞争LC-PUFA生物合成酶，导致n-6 LC-PUFA的产生，包括花生四烯酸(ARA)。我们的目的是量化不含EPA和DHA的饲料中首次投喂的三文鱼中ALA的内源EPA和DHA的产量，并确定饲料LA和ALA/LA比例对LC-PUFA产量的影响。用亚麻籽油和向日葵籽油配制成ALA：LA约为3：1、1：1和1：3的三种饲料饲养鲑鱼22周。在ALA：LA为3：1、1：1和1：3的饲料中，n-3LC-PUFA的内源产量分别为每克鱼5.9、4.4和2.8 mg，n-6 LC-PUFA的内源产量分别为0.2、0.5和1.4 mg/g鱼。随着饲料ALA/La比例的降低，n-3：n-6 LC-PUFA产量比由27.4降至2.0，DHA：EPA比例增加，EPA：Ara和DHA/Ara比例降低。结果表明，在ALA：LA为1的条件下，鲑鱼鱼苗/PARR的日产量约为28μg n-3LC-PUFA/g鱼，DHA/EPA为3.4，n-3LC-PUFA的产量是n-6LC-PUFA的近9倍。降低饲料ALA/La比例降低n-3LC-PUFA产量和EPA/Ara及DHA/Ara比值，但增加n-6LC-PUFA产量和DHA/EPA比值。
Atlantic salmon (Salmo salar) possess enzymes required for the endogenous biosynthesis of n-3 long-chain PUFA (LC-PUFA), EPA and DHA, from α-linolenic acid (ALA). Linoleic acid (LA) competes with ALA for LC-PUFA biosynthesis enzymes leading to the production of n-6 LC-PUFA, including arachidonic acid (ARA). We aimed to quantify the endogenous production of EPA and DHA from ALA in salmon fed from first feeding on diets that contain no EPA and DHA and to determine the influence of dietary LA and ALA:LA ratio on LC-PUFA production. Salmon were fed from first feeding for 22 weeks with three diets formulated with linseed and sunflower oils to provide ALA:LA ratios of approximately 3:1, 1:1 and 1:3. Endogenous production of n-3 LC-PUFA was 5·9, 4·4 and 2·8 mg per g fish and that of n-6 LC-PUFA was 0·2, 0·5 and 1·4 mg per g fish in salmon fed diets with ALA:LA ratios of 3:1, 1:1 and 1:3, respectively. The ratio of n-3:n-6 LC-PUFA production decreased from 27·4 to 2·0, and DHA:EPA ratio increased and EPA:ARA and DHA:ARA ratios decreased, as dietary ALA:LA ratio decreased. In conclusion, with a dietary ALA:LA ratio of 1, salmon fry/parr produced about 28 μg n-3 LC-PUFA per g fish per d, with a DHA:EPA ratio of 3·4. Production of n-3 LC-PUFA exceeded that of n-6 LC-PUFA by almost 9-fold. Reducing the dietary ALA:LA ratio reduced n-3 LC-PUFA production and EPA:ARA and DHA:ARA ratios but increased n-6 LC-PUFA production and DHA:EPA ratio.