A New Method for Determining the Emulsion Stability Index by Backscattering Light Detection
A New Method for Determining the Emulsion Stability Index by Backscattering Light Detection
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DOI：
10.1111/jfpe.12078
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发表时间：
2014-06-01
影响因子：
3
通讯作者：
Chang, Pahn-Shick
中科院分区：
文献类型：
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作者：
Choi, Seung Jun;Won, Jong Won;Chang, Pahn-Shick
在本研究中，背散射（BS）方法被开发为一个简单和灵敏的工具，用于测定乳液稳定性指数（ESI）的背散射光检测。反向散射光检测方法使用乳剂扫描技术来监测乳剂中的物理行为作为储存时间的函数。通过将蒸馏水和含有吐温60的紫苏油均质化（8，000 rpm，25 ℃，2 min）来制备乳液，并将样品在小瓶中于25 ℃静置孵育11 h。为了确认这种新的方法，在澄清层的BS的总和，从后向散射通量分布在存储期间，进行了比较与ESI测定的容量法。我们的反向散射光检测方法进行了比较，与基于乳化剂含量的容量法。乳化剂浓度为200、300、400和500 mol时，随着ESI的降低，澄清层中的BS值也随之降低，相关系数分别为0.999、0.993、0.998和0.998。无论乳液中的乳化剂含量如何，通过将澄清层的BS总和与容量法测定的ESI作图计算的拟合曲线的R2值显示出强线性关系（R2=0.953）。我们的研究结果表明，背散射光检测方法是一种新的工具，定量测定的ESI在乳液体系。实际应用可接受的乳液表征方法是通过测量乳化速率和/或油滴直径来确定乳液稳定性。虽然油滴直径的测定提供了关于乳状液稳定性的各种信息，但它需要基于动态光散射或激光衍射分析的昂贵仪器。另一方面，乳状液分层是用肉眼监测乳状液层和浆液层之间的边界高度的变化。然而，在许多情况下，乳状液的状况（如其颜色、粘度和含油量等）妨碍了边界的清晰阅读。在这项研究中描述的背散射光检测可能是一个很有前途的方法，使准确的分析乳液的稳定性，而不管上述的限制。
In the present study, the backscattering (BS) method was developed as a simple and sensitive tool for determining the emulsion stability index (ESI) by backscattering light detection. The backscattering light detection method uses emulsion-scanning technology to monitor physical behavior in an emulsion as a function of storage time. Emulsions were prepared by the homogenization (8,000rpm for 2min at 25C) of distilled water and perilla oil containing Tween 60, and the sample was quiescently incubated in a vial at 25C for 11h. To confirm this new methodology, the sum of BS at the clarified layer, obtained from the backscattering flux profiles over the storage period, was compared with the ESI determined by the volumetric method. Our backscattering light detection method was compared with the volumetric method based on the emulsifier content. The sum of BS at the clarified layer decreased as the ESI decreased, with a correlation of 0.999, 0.993, 0.998 and 0.998 for emulsions stabilized with 200, 300, 400 and 500mol of emulsifier, respectively. Regardless of the emulsifier content in the emulsion, the R2 values for the fit curve, calculated by plotting the sum of BS at the clarified layer against the ESI determined by the volumetric method, revealed strong linear relationship (R2=0.953). Our results suggest that the backscattering light detection method is a new tool for the quantitative determination of the ESI in an emulsion system. Practical Applications The acceptable way for the emulsion characterization is the determination of emulsion stability by measuring creaming rate and/or oil droplet diameter. Although the determination of oil droplet diameter provides the various information on the emulsion stability, it needs the expensive instruments based on dynamic light scattering or laser diffraction analysis. On the other hand, creaming is monitoring the change in the height of the boundary between the creamed and serum layers with naked eyes. In many cases, however, the condition of emulsions (such as their color, viscosity, and oil content, etc) prevents the clear reading of the boundary. The backscattering light detection described in this study could be a promising method which enables the accurate analysis of emulsion stability regardless of the limitations mentioned above.