Quantification of taurine in energy drinks using 1H NMR
Quantification of taurine in energy drinks using 1H NMR
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DOI：
10.1016/j.jpba.2013.08.046
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发表时间：
2014-05-01
影响因子：
3.4
通讯作者：
Holzgrabe, Ulrike
中科院分区：
文献类型：
--
作者：
Hohmann, Monika;Felbinger, Christine;Holzgrabe, Ulrike
The consumption of so called energy drinks is increasing, especially among adolescents. These beverages commonly contain considerable amounts of the amino sulfonic acid taurine, which is related to a magnitude of various physiological effects. The customary method to control the legal limit of taurine in energy drinks is LC-UV/vis with postcolumn derivatization using ninhydrin. In this paper we describe the quantification of taurine in energy drinks by H-1 NMR as an alternative to existing methods of quantification. Variation of pH values revealed the separation of a distinct taurine signal in H-1 NMR spectra, which was applied for integration and quantification. Quantification was performed using external calibration (R-2 > 0.9999; linearity verified by Mandel's fitting test with a 95% confidence level) and PULCON. Taurine concentrations in 20 different energy drinks were analyzed by both using H-1 NMR and LC-UV/vis. The deviation between H-1 NMR and LC-UV/vis results was always below the expanded measurement uncertainty of 12.2% for the LC-UV/vis method (95% confidence level) and at worst 10.4%. Due to the high accordance to LC-UV/vis data and adequate recovery rates (ranging between 97.1% and 108.2%), H-1 NMR measurement presents a suitable method to quantify taurine in energy drinks. (C) 2013 Elsevier B.V. All rights reserved.
