Ultraviolet radiation-induced connective tissue changes in the skin of hairless mice.
Ultraviolet radiation-induced connective tissue changes in the skin of hairless mice.
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DOI：
10.1111/1523-1747.ep12261342
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发表时间：
1984-06
期刊：
影响因子：
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通讯作者：
K. Johnston;A. Oikarinen;N. Lowe;J. Clark;J. Uitto
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文献类型：
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作者：
K. Johnston;A. Oikarinen;N. Lowe;J. Clark;J. Uitto
将无毛小鼠（Skh/ hr1）暴露于紫外线A （UVA，峰值辐照度为365 nm）或紫外线B （UVB，峰值辐照度为313 nm）辐射下。这些动物每隔一天接受12次治疗。皮肤结缔组织的变化分别通过测定羟脯氨酸和氨基葡萄糖作为胶原蛋白和弹性蛋白浓度的指示来监测。同时测定了参与胶原生物合成的脯氨酸羟化酶和胶原糖基转移酶的活性。UVA或UVB处理小鼠的弹性蛋白浓度显著增加。uva处理小鼠的胶原蛋白浓度不受影响，但反映胶原合成能力的脯氨酸羟化酶活性降低。胶原糖基转移酶活性不变。体外用UVA照射纯化的人脯氨酰羟化酶，在高剂量下，酶活性降低，但UVB没有影响。结果表明，皮肤结缔组织的生物化学的决定性变化可以诱导暴露于紫外线照射小鼠。
Hairless mice (Skh/ hr1 ) were exposed to ultraviolet A (UVA; peak irradiance at 365 nm), or to ultraviolet B (UVB; peak irradiance at 313 nm) radiation. The animals received 12 treatments on alternate days. Connective tissue changes in the skin were monitored by assaying hydroxyproline and desmosine as an indication of collagen and elastin concentrations, respectively. The activities of prolyl hydroxylase and collagen glucosyl-transferase, enzymes participating in the biosynthesis of collagen, were also assayed. The concentration of elastin was significantly increased in mice treated with UVA or UVB. The concentration of collagen was unaffected by the treatments, but the activity of prolyl hydroxylase, reflecting collagen synthetic capacity, was decreased in UVA-treated mice. The collagen glucosyl-transferase activity was unchanged. Irradiation of purified human prolyl hydroxylase with UVA in vitro decreased the enzyme activity at higher doses, but UVB had no effect. The results indicate that definitive changes in the biochemistry of dermal connective tissues can be induced by exposure of mice to UV irradiation.