RESONANCE RAMAN STUDIES OF PURPLE MEMBRANE
RESONANCE RAMAN STUDIES OF PURPLE MEMBRANE
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DOI：
10.1021/bi00632a029
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发表时间：
1977-01-01
期刊：
影响因子：
2.9
通讯作者：
EBREY, TG
中科院分区：
文献类型：
--
作者：
ATON, B;CALLENDER, RH;EBREY, TG
利用流动技术测量了盐生盐杆菌（Halobacterium halobium）光适应紫膜PM 568和M 412的共振拉曼光谱。为了比较的目的，拉曼光谱的模型发色团，全反式和13-顺式的视网膜正丁胺，质子化和未质子化的席夫碱，也得到了。与以前的工作一致，拉曼数据表明，视网膜发色团连接到紫膜蛋白通过质子化的席夫碱的情况下的PM 568和未质子化的席夫碱的M412的形式。这两种形式的颜色调节的基本机制似乎是电子离域。这两种形式的光谱特征相互不同，与模型化合物的光谱特征不同。
The individual resonance Raman spectra of the PM568 and M412 forms of light-adapted purple membrane from Halobacterium halobium were measured using the newly developed flow technique. For comparison purposes, the Raman spectra of the model chromophores, all-trans- and 13-cis retinal n-butylamine, both as protonated and unprotonated Schiff bases, were also obtained. In agreement with previous work, the Raman data indicate that the retinal chromophore is linked to the purple membrane protein via a protonated Schiff base in the case of the PM568 and an unprotonated Schiff base for the M412 form. The basic mechanism for color regulation in both forms appears to be electron delocalization. The spectral features of the 2 forms are different from each other and different from the model compound spectra.