Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly.
Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly.
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DOI：
10.1088/0957-4484/23/49/495304
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发表时间：
2012-12-14
期刊：
影响因子：
3.5
通讯作者：
Stockley PG
中科院分区：
文献类型：
--
作者：
White SJ;Johnson S;Szymonik M;Wardingley RA;Pye D;Davies AG;Wälti C;Stockley PG
近年来，人们利用各种生物支架制作了许多纳米级的器件和材料。然而，事实证明，将这些器件和材料连接到现有电路和有序阵列是有问题的。在这里，我们描述了一个简单的解决方案，使用多肽卷曲杂二聚体酸：碱的自组装将M13噬菌体颗粒固定到有图案的金表面的特定位置。表面等离子体共振表明，游离的酸性多肽可以组装到碱衍生物的表面上。然后，我们在M13的Pix外壳蛋白上展示了酸性多肽，并表明这些噬菌体颗粒允许形成卷曲的卷曲，从而导致特定的表面附着。酸：游离肽和噬菌体展示酸的固定化碱基亲和力似乎相似。最后，我们制作了两个金电极，间隔200 nm，其中一个涂上了碱，这使得M13：酸可以定位在功能化的电极上。
Numerous nanoscale devices and materials have been fabricated in recent years using a variety of biological scaffolds. However, the interfacing of these devices and materials into existing circuits and ordered arrays has proved problematic. Here, we describe a simple solution to this problem using self-assembly of the peptide coiled-coil heterodimer ACID:BASE to immobilize M13 bacteriophage particles to specific locations on a patterned gold surface. Surface plasmon resonance demonstrated that free ACID peptides will assemble onto a surface derivatized with BASE. We then displayed the ACID peptide on the pIX coat protein of M13 and showed that these phage particles permit formation of the coiled-coil resulting in specific surface attachment. The ACID:immobilized BASE affinities appear to be similar for free peptide and phage-displayed ACID. Finally, we fabricated two gold electrodes, separated by a 200 nm gap, coated one of them with BASE and showed that this allows localization of the M13:ACID onto the functionalized electrode.