The Gα subunit BCG1, the phospholipase C (BcPLC1) and the calcineurin phosphatase co-ordinately regulate gene expression in the grey mould fungus Botrytis cinerea
The Gα subunit BCG1, the phospholipase C (BcPLC1) and the calcineurin phosphatase co-ordinately regulate gene expression in the grey mould fungus Botrytis cinerea
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DOI：
10.1111/j.1365-2958.2008.06105.x
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发表时间：
2008-03-01
影响因子：
3.6
通讯作者：
Tudzynski, Bettina
中科院分区：
文献类型：
--
作者：
Schumacher, Julia;Viaud, Muriel;Tudzynski, Bettina
G α亚基BCG 1对灰霉病菌Botrytis cinerea的致病性至关重要。几个过程，如从原发性感染到继发性侵入性生长的过渡和植物毒素葡萄球菌的产生是由BCG 1通过cAMP非依赖性途径调节的。我们最近的发现，葡萄球菌生物合成基因属于钙/钙调神经磷酸酶依赖性基因组的建议，第一次这个G α亚基和钙调神经磷酸酶信号通路之间的连接。为了研究BCG 1和钙调神经磷酸酶对基因的共同调节是否是一个共同特征，使用cDNA宏阵列方法比较野生型和Delta bcg 1突变体的基因表达模式，未处理或用钙调神经磷酸酶抑制剂环孢菌素A处理。我们确定了三组基因，其表达受到BCG 1和钙调神经磷酸酶的调控，或仅受到其中一种的调控。在BCG 1/钙调神经磷酸酶共调节基因中，我们发现了一个新的基因簇，编码一种未知的聚酮次级代谢产物。此外，我们首次在植物病原性真菌中发现，磷脂酶C（BcPLC 1）是BCG 1和钙调神经磷酸酶依赖性信号通路的一个组成部分，因为几个BCG 1和钙调神经磷酸酶依赖性基因在BcPLC 1敲低突变体中下调。
The G alpha subunit BCG1 is essential for pathogenicity of the grey mould fungus Botrytis cinerea. Several processes such as the transition from primary infection to secondary invasive growth and the production of the phytotoxin botrydial are regulated by BCG1 via a cAMP-independent pathway. Our recent finding that the botrydial biosynthesis genes belong to the group of Ca2+/calcineurin-dependent genes suggested for the first time a connection between this G alpha subunit and the calcineurin signalling pathway. To investigate whether this co-regulation of genes by BCG1 and calcineurin is a common feature, a cDNA macroarray approach was used to compare the gene expression pattern of the wild-type and the Delta bcg1 mutant, non-treated or treated with the calcineurin inhibitor cyclosporin A. We identified three sets of genes whose expression was regulated either by both BCG1 and calcineurin, or only by one of them. Among the BCG1/calcineurin-co-regulated genes, we found a new gene cluster coding for a yet unknown polyketide secondary metabolite. Furthermore, we show for the first time in a phytopathogenic fungus that the phospholipase C (BcPLC1) is a component of the BCG1- and calcineurin-dependent signalling pathway as several BCG1- and calcineurin-dependent genes were downregulated in bcplc1 knock-down mutants.