Label-free detection of allergens in food via surfactant-induced signal amplification using a field effect transistor-based biosensor
Label-free detection of allergens in food via surfactant-induced signal amplification using a field effect transistor-based biosensor
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DOI：
10.1016/j.snb.2017.07.187
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发表时间：
2018-01-01
影响因子：
8.4
通讯作者：
Osaka, Tetsuya
中科院分区：
文献类型：
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作者：
Hideshima, Sho;Saito, Mai;Osaka, Tetsuya
近年来，食品过敏相关的食品安全问题越来越受到全球的关注。应开发一种简便、灵敏的检测方法来检测食品中微量的过敏原。在此，我们提出了一种基于无标签场效应晶体管（FET）的生物传感系统，通过表面活性剂诱导的信号放大来检测荞麦致敏蛋白BWp16。BWp16可以通过与阴离子表面活性剂十二烷基硫酸钠（SDS）偶联来检测，因为这种偶联增强了蛋白质的净电荷，足以被FET生物传感器检测到。SDS偶联后反应明显，而与阳离子或非离子表面活性剂偶联后反应减弱或保持不变，说明SDS偶联维持了目标过敏原的抗体识别能力，有助于增强传感器的反应。荧光光谱测量显示SDS分子与靶致敏蛋白BWp16成功偶联，导致其净电荷增加。此外，我们证明了FET生物传感器能够在食品安全分析所需的浓度水平下特异性检测食品中的过敏原，这表明它可以作为工业和国内的替代食品过敏原分析仪。(C) 2017年作者。Elsevier B.V.出版
In recent years, food safety concerning food allergy has received increasing attention globally. A simple and sensitive detection method should be developed to measure trace amounts of allergens in foods. Here, we propose a label-free field effect transistor (FET)-based biosensing system for the detection of a buckwheat allergenic protein, BWp16, by surfactant-induced signal amplification. BWp16 could be detected by coupling with an anionic surfactant, sodium dodecyl sulfate (SDS), as this coupling enhanced the net charge of the protein, enough to be detected by FET biosensors. A significant response was observed when the allergen was coupled with SDS, while the responses were decreased or unchanged when it was coupled with a cationic or non-ionic surfactant, suggesting that the SDS coupling maintains the antibody recognition ability of the target allergen, and it would be useful to enhance the sensor responses. The fluorescence spectroscopic measurement revealed that the SDS molecules were successfully coupled with target allergenic protein BWp16, resulting in an increase in its net charge. Furthermore, we demonstrated that the FET biosensor enables specific detection of the allergen in food at the desired concentration levels for food safety analysis, suggesting that it could be used as an alternative food allergen analyzer, both industrially and domestically. (C) 2017 The Authors. Published by Elsevier B.V.