QTL analysis of crown gall disease resistance in apple: first plant R gene candidates effective against Rhizobium rhizogenes (Ti)
QTL analysis of crown gall disease resistance in apple: first plant R gene candidates effective against Rhizobium rhizogenes (Ti)
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苹果冠瘿病抗性QTL分析：对根瘤菌有效的首株R基因候选物
DOI：
10.1007/s11295-021-01508-9
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发表时间：
2021-06-01
影响因子：
2.4
通讯作者：
Abe, Kazuyuki
中科院分区：
文献类型：
--
作者：
Moriya, Shigeki;Iwanami, Hiroshi;Abe, Kazuyuki
在苹果（Malus spp.）中，冠瘿病是由根瘤菌Rhizobium radiotherapy（Ti）和R.生根瘤菌（Ti），可能严重。培育抗根癌病砧木是防治该病的有效途径。在这项研究中，我们使用的全同胞F-1人口来自'JM 7'（敏感）x Sanashi 63（抗）杂交，以确定数量性状基因座（QTL），控制在日本的苹果生产地区发现的肿瘤诱导根瘤菌菌株的抗性。在3个连锁群中检测到3个与冠瘿病抗性相关的稳定QTL。对桃品种CG 8331、长野1号和长野2号的抗性QTL共定位于Sanashi 63的LG 2的中部，在此位置已鉴定出抗根癌基因Cg（本研究中更名为Rrr 1）。在“JM 7”中，分别在LG 11和LG 15上检测到了对ARAT-001、ARAT-002和和野2的抗性QTL和对ARAT-001的抗性QTL。Rrr 1基因的精细定位和从两个细菌人工染色体（BAC）克隆获得的重叠群的核苷酸测序将Rrr 1基因界定为跨越217 kb的区域。从该区域的计算机基因预测确定了四个编码Toll-白细胞介素-1受体/核苷酸结合位点/富含亮氨酸重复序列（TIR-NBS-LRR）类植物抗性基因的基因。
In apple (Malus spp.), crown gall disease, caused by the bacterial pathogens Rhizobium radiobacter (Ti) and R. rhizogenes (Ti), can be severe. To control the disease, breeding of apple rootstocks that exhibit crown gall resistance is a promising approach. In this study, we used a full-sib F-1 population derived from a 'JM7' (susceptible) x Sanashi 63 (resistant) cross to identify quantitative trait loci (QTLs) that control resistance to tumour-inducing Rhizobium isolates found in apple production areas in Japan. Three stable QTLs, associated with a wide range of crown gall resistances, were identified in three linkage groups (LGs). QTLs for resistance to isolates Peach CG8331, Nagano 1 and Nagano 2 co-localised at the middle of LG 2 in Sanashi 63, where the crown gall resistance gene Cg (renamed as Rrr1 in this study) was previously identified. Similarly, in 'JM7', QTLs were identified on LG 11 for resistance to isolates ARAT-001, ARAT-002 and Kazuno 2, and on LG 15 for resistance to isolate ARAT-001. Fine-mapping of Rrr1 and nucleotide sequencing of a contig obtained from two bacterial artificial chromosome (BAC) clones delimited the Rrr1 gene to a region spanning 217 kb. In silico gene prediction from the region identified four genes encoding the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR-NBS-LRR) class plant resistance genes.