Identification and expression analysis of suppressors of cytokine signaling (SOCS) of Japanese flounder Paralichthys olivaceus.
Identification and expression analysis of suppressors of cytokine signaling (SOCS) of Japanese flounder Paralichthys olivaceus.
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DOI：
10.1016/j.fsi.2016.09.018
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发表时间：
2016-11
影响因子：
4.7
通讯作者：
Kittipong Thanasaksiri;I. Hirono;H. Kondo
中科院分区：
文献类型：
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作者：
Kittipong Thanasaksiri;I. Hirono;H. Kondo
细胞因子信号转导抑制因子(SoCs)家族成员是免疫系统的关键调节因子，尤其是细胞因子的作用，目前已在许多鱼类中发现。在本研究中，我们鉴定了8种牙鲆SOCS蛋白(CISH、SOCS1a、SOCS1b、SOCS3a、SOCS3b、SOCS5、SOCS6和SOCS9)，并分析了它们在迟缓爱德华氏菌聚(I：C)和福尔马林灭活细胞(FKC)注射后的mRNA表达。8种SOCS基因在所有受检组织中均有表达。在15℃和25℃条件下，用Poly(I：C)刺激比目鱼，可影响CISH、SOCS1a、SOCS1b和SOCS3a基因表达。注射FKC后，所有SOCS基因的mRNA水平均发生明显变化。SOCS1a、SOCS1b、SOCS3a和SOCS3b基因在3HPI、12HPI和24HPI时显著上调。SOCS5和SOCS6基因在3HPI时显著下调。SOCS9基因在12HPI时显著上调。这些结果表明，所有8个SOCS基因都参与了免疫反应，并且CISH、SOCS1和SOCS3基因具有不同于其他SOCS成员的功能。
Suppressor of cytokine signaling (SOCS) family members are key regulators of the immune system, particularly cytokine action, and have now been discovered in a number of fish species. Here we identified eight SOCS proteins (CISH, SOCS1a, SOCS1b, SOCS3a, SOCS3b, SOCS5, SOCS6 and SOCS9) in the Japanese flounder and analyzed their mRNA expressions after injection of poly (I:C) and formalin-killed cells (FKC) ofEdwardsiella tarda. The expressions of all eight SOCS genes were detected in all the tissues examined. Stimulation of Japanese flounder reared at 15 or 25 °C with poly (I:C) affected the gene expressions of CISH, SOCS1a, SOCS1b and SOCS3a. All SOCS genes mRNA levels were significantly changed after FKC injection. Significant up-regulation of SOCS1a, SOCS1b, SOCS3a and SOCS3b genes was detected at 3, 12 and 24 hpi. SOCS5 and SOCS6 genes were significantly down-regulated at 3 hpi. SOCS9 gene was significantly up-regulated at 12 hpi. These results suggest that all eight of the SOCS genes are involved in immune responses, and that the CISH, SOCS1 and SOCS3 genes have functions distinct from those of the other SOCS members.