Peptide Fractionation and Free Radical Scavenging Activity of Zein Hydrolysate
Peptide Fractionation and Free Radical Scavenging Activity of Zein Hydrolysate
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DOI：
10.1021/jf9028656
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发表时间：
2010-01-13
影响因子：
6.1
通讯作者：
Chen, Jie
中科院分区：
文献类型：
--
作者：
Tang, Xueyan;He, Zhiyong;Chen, Jie
采用凝胶过滤、超滤和反相高效液相色谱法对玉米醇溶蛋白水解物（ZH）进行分离，并测定不同肽段对2，2 '-偶氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS（中心点+））、1，1-二苯基-2-苦肼基（DPPH中心点）和超氧阴离子（O-2（中心点-））自由基的清除活性，以确定其抗氧化能力。结果表明，ZH组分对水溶性自由基（ABTS（中心点+））的稳定能力与肽的大小无关，而对醇溶性自由基（DPPH中心点）和O-2（中心点-）的稳定能力则与肽的分子量有关。抗氧化肽
Alcalase-treated zein hydrolysate (ZH) was separated by gel filtration, ultrafiltration, and reversed-phase HPLC, and the scavenging activities for 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(center dot+)), 1,1-diphenyl-2-picrylhydrazyl (DPPH center dot), and superoxide anion (O-2(center dot-)) radicals of different peptide fractions were measured to establish the antioxidant efficacy. Results showed that the ability to stabilize water-soluble free radicals (ABTS(center dot+)) by ZH components was insensitive to the peptide size, whereas that against ethanol-soluble free radicals (DPPH center dot) and O-2(center dot-) was molecular weight dependent. Antioxidative peptides of