Distribution and genetic diversity of genes involved in quorum sensing and prodigiosin biosynthesis in the complete genome sequences of Serratia marcescens
Distribution and genetic diversity of genes involved in quorum sensing and prodigiosin biosynthesis in the complete genome sequences of Serratia marcescens
复制标题
粘质沙雷氏菌全基因组序列中群体感应和灵菌红素生物合成相关基因的分布和遗传多样性
DOI：
10.1093/gbe/evz046
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发表时间：
2019
影响因子：
3.3
通讯作者：
T. Morohoshi
中科院分区：
文献类型：
--
作者：
R. Sakuraoka;T. Suzuki;T. Morohoshi
群体感应是一种依赖于细胞密度的基因表达调控。N-酰基-L-高丝氨酸内酯是革兰氏阴性菌中主要的群体感应信号分子，由鲁西家族蛋白合成。沙雷氏属是一种红色色素的生产者，它的生物合成依赖于色素基因簇。有些沙雷菌素通过AHL介导的群体感应来调节灵芝菌素的产生，而有些红色素的沙雷菌素则没有群体感应系统。此外，从自然和临床环境中也分离到了不产生灵芝菌素的无色素粘质沙雷氏菌。在本研究中，我们旨在揭示群体感应基因和猪基因簇在OFS全基因组序列中的分布和遗传多样性。粘质的。我们之前已经证明了这一点。MarcescensAS-1通过AHL介导的群体感应调节灵芝菌素的产生。我们对AS-1的基因组进行了测序，并与AS-1和其他34株OFS的全基因组进行了比较。粘质的。该同源基因存在于25个完整的基因组序列中。推导出的氨基酸序列可分为三个系统发育类。相比之下，猪基因簇存在于七星鸟的基因组中。Marcescens3株，仅AS-1和N4-5两株在基因组中既含有luxI同源基因簇，又含有pG基因簇。因此，假设灵芝菌素的生产及其通过群体感应进行的调节对于OFS的生命周期并不是必不可少的。粘质的。
Quorum sensing is a cell density-dependent regulation of gene expression.N-acyl-l-homoserine lactone (AHL) is a major quorum-sensing signaling molecule in gram-negative bacteria and synthesized by the LuxI family protein. The genusSerratiais known as a producer of the red pigment, prodigiosin, whose biosynthesis is dependent on thepiggene cluster. SomeSerratiastrains regulate prodigiosin production via AHL-mediated quorum sensing, whereas there is red-pigmentedSerratiastrains without quorum-sensing system. In addition, nonpigmentedSerratia marcescens, which does not produce prodigiosin, has also been isolated from natural and clinical environments. In this study, we aim to reveal the distribution and genetic diversity of quorum-sensing genes andpiggene cluster in the complete genome sequences ofS. marcescens. We previously demonstrated thatS. marcescensAS-1 regulates the production of prodigiosin via AHL-mediated quorum sensing. We sequenced the genomes of AS-1 and compared with the complete genomes of AS-1 and the other 34 strains ofS. marcescens. TheluxIhomolog was present on 25 complete genome sequences. The deduced amino acid sequences of theluxIhomolog were divided into three phylogenetic classes. In contrast, thepiggene cluster was present in the genome of sevenS. marcescensstrains and only two strains, AS-1 and N4-5 contained both theluxIhomolog andpiggene cluster in their genome. It is therefore assumed that prodigiosin production and its regulation by quorum sensing are not essential for the life cycle ofS. marcescens.